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Biotechnology & Biotechnological Equipment Volume 33, 2019 - Issue 1
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Articles

Insulin-like growth factor-1 inhibits the apoptosis of rat gastric smooth muscle cells cultured under high glucose condition through PI3K-Akt-PKC-Ca2+ pathway

Xue-Sen FangDepartment of Histology and Embryology, College of Medicine, Yanbian University, Yanji, Jilin, PR China
,
Mo-Han ZhangDepartment of Histology and Embryology, College of Medicine, Yanbian University, Yanji, Jilin, PR China
,
Xiang-Zi ZhangDepartment of Histology and Embryology, College of Medicine, Yanbian University, Yanji, Jilin, PR China
,
Jun-Yu GuoDepartment of Histology and Embryology, College of Medicine, Yanbian University, Yanji, Jilin, PR China
&
Zheng JinDepartment of Histology and Embryology, College of Medicine, Yanbian University, Yanji, Jilin, PR ChinaCorrespondence[email protected]
Pages 456-464 | Received 30 Jul 2018, Accepted 18 Feb 2019, Published online: 03 Apr 2019

Figures & data

Figure 1. A: Effect of IGF-1 on rat gastric smooth muscle cells: PKC activity detected by ELISA (A); intracellular Ca2+ concentration detected by confocal laser-scanning microscopy (B); cell apoptosis detected by flow cytometry (C). ***p＜0.001；**p＜0.01；*p＜0.05.

Figure 1. A: Effect of IGF-1 on rat gastric smooth muscle cells: PKC activity detected by ELISA (A); intracellular Ca2+ concentration detected by confocal laser-scanning microscopy (B); cell apoptosis detected by flow cytometry (C). ***p＜0.001；**p＜0.01；*p＜0.05.

Figure 2. Effect of IGF-1 on protein expression of PKCα (A), p-PKCα (B), PKCβ1 (C), p-PKCβ1 (D), p-Akt (E) and PI3K (F) in rat gastric smooth muscle cells after 24 and 48 h of culture. Western blot analysis. ***p < 0.001; **p < 0.01; *p < 0.05.

Figure 2. Effect of IGF-1 on protein expression of PKCα (A), p-PKCα (B), PKCβ1 (C), p-PKCβ1 (D), p-Akt (E) and PI3K (F) in rat gastric smooth muscle cells after 24 and 48 h of culture. Western blot analysis. ***p < 0.001; **p < 0.01; *p < 0.05.

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