P38 mitogen-activated protein kinase inhibition attenuates mechanical stress induced lung injury via up-regulating AQP5 expression in rats

Figures & data

Table 1. Scoring criteria for lung injury.

Score	Alveolar wall thickening	Inflammatory cell infiltration	Pulmonary hyaline membrane formation	Atelectasis change
0	No change	No change	No change	No change
1	Alveolar wall thickness was less than 1/5 of the alveolar cavity diameter	Infiltration of inflammatory cells showed lesions in point-like distribution and only around the microvessels	Formation of pulmonary hyaline membrane only focally distributed in a single alveolar cavity	Atelectasis area was less than 1/4 of pathological section of the entire lung tissue
2	Between mild and severe	Between mild and severe	Between mild and severe	Between mild and severe
3	Alveolar wall thickness was greater than 1/3 of the alveolar cavity diameter	Infiltration of inflammatory cells was in patch-like distribution and had permeated into the alveolar cavity	Pulmonary hyaline membrane formed continuous distribution in a large area	Atelectasis area was greater than 1/2 of pathological section of the entire lung tissue

Figure 1. Pathological changes of lung tissue in different groups. Representative H&E staining results (A) and lung injury score (B).

Note: Scale bar = 16 μm; ^ap <0.05 vs. Group C; ^b p <0.05 vs. Group S; ^c p <0.05 vs. Group L. Data are means ± SD, n = 10 in each group.

Figure 2. Expression of P38 MAPK and p-P38 MAPK in different groups.

Note: Error bars represent S.D. of 10 rats ^a p < 0.05 vs. Group C; ^b p < 0.05 vs. Group S; ^c p < 0.05 vs. Group L.

Figure 3. Expression of AQP5 in lung tissues of different groups examined by immunohistochemical analysis (A) and western blot assay (B).

Note: Scale bar: 16 μm. Error bars represent S.D. of 10 rats. ^a p < 0.05 vs. Group C; ^bp < 0.05 vs. Group S; ^cp < 0.05 vs. Group L.

Figure 4. W/D (A) and PMN counts (B) in different groups.

Note: ^a p < 0.05 vs. Group C; ^b p < 0.05 vs. Group S; ^c p < 0.05 vs. Group L. Data are shown means ± SD, n = 10 in each group.

Figure 5. TNF-α (A) and IL-6 (B) in different groups.

Note: ^a p < 0.05 vs. Group C; ^b p < 0.05 vs. Group S; ^cp < 0.05 vs. Group L. Data are means ± SD, n = 10 in each group.