Quantity, diversity and complexity of subgingival microorganisms in children with plaque-induced gingivitis

Figures & data

Table 1. Microorganisms tested, grouped according to Socransky.

Aggreggatibacter actinomycetemcomitans	Purple complex
Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia	Red complex
Prevotela intermedia, Peptostreptococcus (micromonas) micros, Fusobacterium nuleatum, Eubacterium nodatum	Orange complex
Capnocytophaga gingivalis	Green complex

Figure 1. Average values of OHI – GV in subjects.

Table 2. Degree of severity of gingival inflammation.

Groups PBI	Healthy		Gingivitis		Ind T-test
	N	Mean ± SD	N	Mean ± SD
Total	30	0.21 ± 0.25	30	0.98 ± 0.33	t1,2 = −10.030, p = .000

Table 3. Average quantity of isolated microorganisms.

Microorganisms		Healthy		Gingivitis	Total		Ind T-test
	N	Mean ± SD	N	Mean ± SD	N	%
Aggreggatibacter actinomycetemcomitans	0	0 ± 0	7	2.9 × 10^3 ± 9.6 × 10^3	30	100	–
Porphyromonas gingivalis	0	0 ± 0	8	8.2 × 10^4 ± 4.4 × 10^5	30	100	–
Treponema denticola	3	1.4 × 10^2 ± 6.8 × 10^2	19	7.9 × 10^3 ± 1.4. × 10^4	30	100	t = −2.829, p = .006
Tannerella forsythia	1	3.7 × 10 ± 2 × 10^2	11	3.6 × 10^3 ± 7.9 × 10^3	30	100	t = −2.516, p = .015
Prevotela intermedia	2	1 × 10^2 ± 3.9 × 10^2	9	1.1 × 10^4 ± 3.7 × 10^4	30	100	t = −1.695, p = .095
Peptostreptococcus (micromonas)micros	6	1.3 × 10^2 ± 4.3 × 10^2	15	1.6 × 10^3 ± 2.7 × 10^3	30	100	t = −3.003, p = .004
Fusobacterium nucleatum	21	9.8 × 10^3 ± 2.8 × 10^4	28	3.7 × 10^4 ± 9.1 × 10^4	30	100	t = −1.555, p = .125
Eubacterium nоdаtum	0	0 ± 0	2	1.8 × 10 ± 7.6 × 10	60	100	–
Capnocytophaga gingivalis	29	5.9. × 10^4 ± 9.2 × 10^4	30	2.5 × 10^5 ± 5.2 × 10^5	60	100	t = −1.958, p = .055

All statistically significant difference between the groups are underlined.

Table 4. Microbial associations in healthy children and children with gingivitis.

Microbial associations		Healthy		Gingivitis	T
	N	% ± sp	N	% ± sp
With 1 microorganism	7	23.3 ± 7.72	0	0 ± 0	–
With 2 microorganisms	16	50.3 ± 9.11	6	20 ± 7.30	t = 2.86
With 3 microorganisms	6	20 ± 7.30	6	20 ± 7.30	t = 0
With 4 microorganisms	1	3.3 ± 3.28	4	13.3 ± 6.21	t = 1.42
With 5 microorganisms	0	0 ± 0	3	10 ± 5.48	–
With 6 microorganisms	0	0 ± 0	7	23.3 ± 7.72	–
With 7 microorganisms	0	0 ± 0	4	13.3 ± 6.21	–
Total	30	100	30	–	–

Pearson Chi-square = 27.345, Sig = .000.

Pearson’s R = 6.654, Sig = .000.

Spearman corr = 6.754, Sig = .000.

All statistically significant difference between the groups are underlined.

Figure 2. Microbial associations according to the number of microorganisms (MO) in healthy children.

Figure 3. Microbial associations of the microorganisms (MO) in the subgingival microflora of children with plaque-induced gingivitis.