Types of microorganisms in proximal caries lesion and ozone treatment

Figures & data

Table 1. Frequency and species affiliation of the microorganisms isolated from deep proximal caries lesions.

		Samples* from teeth
Isolated microorganisms		Sample N1 n = 22	Sample N2 n = 22
Group 1 - Oral streptococci - 20 (90.91%)
Mutans group - 4 (18.18%)	Streptococcus mutans	3 (++++)	–
	Streptococcus cricetus	1 (++++)	–
Mitis group 5 (22.73%)	Streptococcus oralis	1 (++++)	–
	Streptococcus parasanguinis	1 (++++)	–
	Streptococcus sanguinis	2 (++++)	–
	Streptococcus gordonii	1 (++++)	–
Salivarius group 2 (9.09%)	Streptococcus salivarius	1 (++++)	–
	Streptococcus vestibularis	1 (+++)	–
Anginosus group. - 9 (40.90%)	Streptococcus constellatus	3 (++++)	–
	Streptococcus intermedius	4 (++++)	–
	Streptococcus anginosus	2 (++++)	–
Group 2 - Gram-positive cocci - 5 (22.72%)
	Enterococcus faecalis	5 (++++)	–
Group 3 - Peptostreptococcus spp. - 8 (36.36%)
	Peptostreptococcus prevotii	4 (+++)	–
	Peptostreptococcus anaerobius	2 (+++)	–
	Peptostreptococcus magnus	1 (+++)	–
	Peptostreptococcus micros	1 (++++)	–
Group 4 Candida - 8 (36.36%)
	Candida albicans	8 (++++)	–
Group 5 Staphylococсus spp. - 4 (18.18%)
	Staphylococсus epidermidis	3 (+++)	–
	Staphylococсushaemolyticus	1 (+++)	–
Group 6 Gram-negative rods - 3 (13.63%)
	Prevotella intermedia	1 (++++)	–
	Prevotellaoralis	1 (++++)	–
	Prevotellamelaninogenica	1 (++++)	–
Group 7 Leptotrichia - 2 (9.09%)
	Leptotrichia buccalis	2 (++++)	–
Others
	аctinomyces naeslundii - 1 (4.56%)	1 (++++)	–
	Acinetobacter Lwoffii - 1 (4.56%)	1 (++)	–
	Streptococcus bovis - 1 (4.56%)	1 (++)	–
	Corynebacterium аquaticum - 1 (4.56%)	1 (++)	–

* Note: Sample N1 (microorganisms from the caries lesions) and Sample N2 (microorganisms from the prepared cavity after ozone application).

(–), <10³ CFU·mL⁻¹; (+), −10³ CFU·mL⁻¹; (++), 10⁴ CFU·mL⁻¹; (+++), 10⁴–10⁵ CFU·mL⁻¹; (++++), >10⁵ CFU/mL.

Table 2. Kruskal–Wallis analysis for mean of various groups.

Bacterial species
Group*	n	Mean rank	SD
1 2 3 4 5 6 7	22	123.00	0.294
	22	70.50	0.428
	22	81.00	0.492
	22	77.50	0.477
	22	67.00	0.395
	22	63.50	0.351
	22	60.00	0.294

* See Table 1.

Table 3. Comparison between all tested groups of microorganisms using Mann–Whitney test.

	Group* 1 p value	Group 2 p value	Group 3 p value	Group 4 p value	Group 5 p value	Group 6 p value	Group 7 p value
Group 1	–	0.0001*	0.0003*	0.0001*	0.0001*	0.0001*	0.0001*
Group 2	–	–	0.3415	0.5169	0.7236	0.4620	0.2482
Group 3	–	–	–	0.7594	0.1949	0.0967	0.0401
Group 4	–	–	–	–	0.3189	0.1713	0.0770
Group 5	–	–	–	–	–	0.7003	0.4166
Group 6	–	–	–	–	–	–	0.6649

* See Table 1.