Enhancement of xylanase expression by Komagataella phaffii through pexophagy inhibition

Figures & data

Table 1. Primers (Restriction endonuclease sites were marked by bold-underline).

Name	Sequence (5’-3’)	Restriction endonuclease
up-atg30-F	CCGTTTGATAGATCTATGAATTCCACGCTATTCCTCC	Bgl II
up-atg30-R	ATTCTCGAGGATCAAAAATCCCAAGCAAACTG	Xho I
down-atg30-F	ACTGTAATACTAATTAGCCAACC
down-atg30-R	TGGAATTCATAGATCTATCAAACGGTACATTGCCAAGT	Bgl II
atg30-F	CATGAGATCAGATCTTATAGTTGATAAGGTATGAGAA	Bgl II
atg30-R	CCGCCGCGGCTCGAGGTTAAAATCTCCTGTTTGAGCT	Xho I
in- atg30-F	TGGACGACTACGACTATGA
in- atg30-R	GGAAGAGGTGAGGAAGGT
kan-his-F	GCATGACTCGAGATGCGGTAGTTTATCACAGT	Xho I
kan-his-R	GCATGACTCGAGAGGCCGCGATTAAATTCCAA	Xho I

Figure 1. Growth curves of K. phaffii GS115, K. phaffii atg30Δ and K. phaffii atg30Δ complement.

Figure 2. AOX and FDH activities during pexophagy of K. phaffii GS115, K. phaffii atg30Δ and K. phaffii atg30Δ complement. AOX (A) and FDH (B) activity during micropexophagy; AOX (C) and FDH (D) activity during macropexophagy.

Figure 3. Effect of methanol concentration on cell density and xylanase activity of recombinant K. phaffii strains. Cell density (A) and xylanase activity (B) of K. phaffii xynB; Cell density (C) and xylanase activity (D) of K. phaffii xynB/atg30Δ.

Figure 4. Effect of sorbitol and 0.5% methanol addition on cell density and xylanase activity of recombinant K. phaffii strains. Cell density (A) and xylanase activity (B) of K. phaffii xynB; Cell density (C) and xylanase activity (D) of K. phaffii xynB/atg30Δ.

Figure 5. EC and residual sorbitol content during recombinant K. phaffii cultivation with methanol and sorbitol. EC of K. phaffii xynB (A) and K. phaffii xynB/atg30Δ (B); Residual sorbitol (C).