Figures & data
Figure 1. Structure of pEarleyGate-BoMYB vector for plant transformation.
Figure 2. Phylogenetic tree showing the evolutionary relationship and the homology degrees among the MYB amino acid sequences. The estimated genetic distance between sequences is in proportional to the lengths of the horizontal lines connecting one sequence to another. The sequences are of the following MYB transcription factors: Brassica napus (XP_013681390.1), Raphanus sativus (XP_0184687473.1), Gossypium hirsutum (XP_016694638.1), Nicotiana tomentosiformis (NP_001306786), Tarenaya hassleriana (XP_010551826.1), Camelina sativa (XP_010513542.1), Arabidopsis thaliana (NP_176813.1), Arabidopsis lyrata subsp. (XP_002887034.1), and Brassica oleracea (XP_013590812.1).
Figure 3. Alignment of BoMYB protein sequense of Brassica oleracea L.var.acephalawith Brassica napus MYB (GenBank Ac. No. XP_013681390.1), Raphanus sativus MYB (GenBank Ac. No.XP_0184687473.1), Gossypium hirsutum MYB (GenBank Ac. No.XP_016694638.1), Nicotiana tomentosiformis MYB (GenBank Ac. No.NP_001306786), Tarenaya hassleriana MYB (XP_010551826.1), Camelina sativa MYB (XP_010513542.1), Arabidopsis thaliana MYB (NP_176813.1), Arabidopsis lyrata subsp. MYB (XP_002887034.1), and Brassica oleracea MYB(XP_013590812.1). The identical amino acids are shown as white letters on a black background. Alignment within solid line shows R2 and R3 domains. Conservative 13th, 33rd, 53rd, 85th, and 104th amino acids in R2 and R3 domains.
Figure 4. Exterior colour (A), anthocyanin content (B), and BoMYB expression (C) in different tissues of kale.
Figure 5. Expression level of BoMYB in transgenic Arabidopsis thaliana. Cn1, cn2, cn3, cn4, cn5, cn6, cn7, and cn8: different transgenic Arabidopsis plants, respectively.
Figure 6. Morphological characteristics (A) and anthocyanin content (B) in different Arabidopsis plants. Cn1, cn4, cn6, and cn7: different transgenic Arabidopsis plants; WT: Wild-type Arabidopsis plant.
