Isolation, characterization and molecular identification of a halotolerant Bacillus megaterium CTBmeg1 able to grow on halogenated compounds

Figures & data

Figure 1. Sample collection site on the map (latitude: 39°06′30.8N; longitude: 33°18′58.4E) at Tuz Lake, central Turkey).

Figure 2. Morphological properties of strain CTBmeg1. (a) Pure colony of strain CTBmeg1 on 10 mmol/L 2,2-DCP minimal media agar supplemented with 10% NaCl at 30 °C after 9 days of incubation; (b) negative control (plate not supplemented with 2,2-DCP); (c) Gram-staining (observed under 100× oil immersion magnification); (d) SEM micrograph of isolate visualized at 11,000× magnification, 2.0 kV accelerating voltage. Note: Bar: 5μm. Bacterial size was measured using Hitachi SU5020 SEM software.

Table 1. comparison of morphological and biochemical characteristics of CTBmeg1 and other B. megaterium strains.

	Bacillus megaterium
Characteristics	Strain CTBmeg1 (this article)	Strain YJB3Feng et al. [41]	Strain WS24 Zhang et al. [42]	Strain G3 Liu et al. [43]	Strain BJC3.1 Li et al. [44]
Pigmentation	faint yellow	faint yellow	milk white	faint yellow
Opacity	opaque	opaque		opaque
Morphology	long rod shape	long rod shape	short rod shape	short rod shape
Size (μm)	0.74 width 1.96 in length	1.5–2.0 in width 5.2–7.8 in length	0.3–0.5 in width 1.1–1.5 in length	1.2–1.5 in width 1.9–3.7 in length
Motility	+	+	+
Gram staining	+	+	+	+	+
Aeriobic growth	+	+			anaerobic growth
Spore formation	+	+	–		+
Catalase activity	+	+	+		+
Glucose fermentation	acid	acid	acid		acid
Nitrate reduction	–	–			+
Starch hydrolysis	+	+	+		+
Gelatin hydrolysis	–	–
Indole production	–	–
Methyl red	+	+
Voges-Proskauer reaction	–	–			+
Citrate utilization test	–	–
Hydrogen sulfide test	–	–
Urea test	+	+
Phosphotase	–
α metil glucoside	+
Sorbitol	+
D + Trehalose	+
D + Arabinose	+
Oxidase	+
Source	TuzGölü Lake	root tissue	rhizosphere of wheat	root of tobacco	sauce

Figure 3. Phylogenetic tree of B. megaterium strain CTBmeg1. Note: The accession number for each sequence was obtained from NCBI while an outgroup used the sequence of Pseudomonas putida KMBU4308.

Figure 4. Growth of B. megaterium CTBmeg1 at varying growth conditions. (a) 2,2-DCP concentrations; (b) pH values; (c) temperature degrees; (d) rotation speed.

Figure 5. Growth curve of B. megaterium CTBmeg1 at different concentrations of NaCl.

Figure 6. Agarose gel showing partial DehCTBmeg1 gene amplification using Groups I dehalogenase primers. Note: Lane1: minisizer 50 bp DNA lader; Lane2: dehI-f, dehI-r and CTBmeg1 genomic DNA (± 250bp).

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