The first transcriptome sequencing and analysis of the endangered plant species Picea neoveitchii Mast. and potential EST-SSR markers development

Figures & data

Table 1. Summary of P. neoveitchii unigenes.

Unigene length	Total number	Percentage
200-300	27,133	41.10%
300-500	15,959	24.17%
500-1000	10,085	15.28%
1000-2000	8,506	12.88%
>2000	4,339	6.57%
Total number	66,022
Total length	44,098,753
N50 length	1,194
Mean length	668

Figure 1. Species distribution of the top BLAST hits in the NR database.

Table 2. Functional annotation of the P. neoveitchii transcriptome.

Database	Unigene	Percentage
NR	41,011	62.12%
Swissprot	30,924	46.84%
GO	25,842	39.14%
COG	15,737	23.84%
KEGG	12,302	18.63%
All-annotated	42,441	64.28%

Figure 2. Gene Ontology (GO) classification of assembled unigenes in P. neoveitchii.

Figure 3. Classification of clusters of orthologous groups (COG).

Figure 4. Number of P. neoveitchii unigenes that may be involved in the biosynthesis pathways of cellulose (A) and lignin (B).

Figure 5. Putative transcription factors encoded by unigenes in P. neoveitchii.

Table 3. Length distribution of EST-SSRs based on the number of repeat units.

Number of repeat units	Di-	Tri-	Tetra-	Penta-	Hexa-	Total	Percentage
5	—	397	7	6	4	414	46.67%
6	121	135	1	1	3	261	29.43%
7	50	53	0	0	0	103	11.61%
8	40	14	0	0	1	55	6.20%
9	16	1	0	0	0	17	1.92%
10	11	0	0	0	0	11	1.24%
>10	25	1	0	0	0	26	2.93%