2,647
Views
23
CrossRef citations to date
0
Altmetric
Articles

Antifungal activity and functional components of cell-free supernatant from Bacillus amyloliquefaciens LZN01 inhibit Fusarium oxysporum f. sp. niveum growth

ORCID Icon, , , &
Pages 1042-1052 | Received 05 Mar 2019, Accepted 21 Jun 2019, Published online: 05 Jul 2019

Figures & data

Figure 1. Curve of B. amyloliquefaciens LZN01 growth (A) and the effects of the CFS on Fon mycelial growth (B). 12 h, 24 h, 36 h, 48 h, 60 h and 72 h: the CFS from B. amyloliquefaciens LZN01 in relation to the incubation time.

Figure 1. Curve of B. amyloliquefaciens LZN01 growth (A) and the effects of the CFS on Fon mycelial growth (B). 12 h, 24 h, 36 h, 48 h, 60 h and 72 h: the CFS from B. amyloliquefaciens LZN01 in relation to the incubation time.

Figure 2. SEM and TEM micrographs of Fon conidia treated with the CFS from B. amyloliquefaciens LZN01: (A) 50% CFS from LB medium at 72 h (SEM), (B) 50% CFS from B. amyloliquefaciens LZN01 at 72 h (SEM), (C) 75% CFS from LB medium at 72 h (SEM), (D) 75% CFS from B. amyloliquefaciens LZN01 at 72 h (SEM), (E) 50% CFS from LB medium at 72 h (TEM), (F) 50% CFS from B. amyloliquefaciens LZN01 at 72 h (TEM), (G) 75% CFS from LB medium at 72 h (TEM), (H) 75% CFS from B. amyloliquefaciens LZN01 at 72 h (TEM).

Figure 2. SEM and TEM micrographs of Fon conidia treated with the CFS from B. amyloliquefaciens LZN01: (A) 50% CFS from LB medium at 72 h (SEM), (B) 50% CFS from B. amyloliquefaciens LZN01 at 72 h (SEM), (C) 75% CFS from LB medium at 72 h (SEM), (D) 75% CFS from B. amyloliquefaciens LZN01 at 72 h (SEM), (E) 50% CFS from LB medium at 72 h (TEM), (F) 50% CFS from B. amyloliquefaciens LZN01 at 72 h (TEM), (G) 75% CFS from LB medium at 72 h (TEM), (H) 75% CFS from B. amyloliquefaciens LZN01 at 72 h (TEM).

Figure 3. Confocal laser scanning micrographs of Fon conidia and mycelia treated with the CFS from B. amyloliquefaciens LZN01 and LB medium: CFS from B. amyloliquefaciens LZN01 for 12 h (A), 24 h (B), 36 h (C) and 48 h (D) vs. CFS from LB medium for 12 h (E), 24 h (F), 36 h (G) and 48 h (H).

Figure 3. Confocal laser scanning micrographs of Fon conidia and mycelia treated with the CFS from B. amyloliquefaciens LZN01 and LB medium: CFS from B. amyloliquefaciens LZN01 for 12 h (A), 24 h (B), 36 h (C) and 48 h (D) vs. CFS from LB medium for 12 h (E), 24 h (F), 36 h (G) and 48 h (H).

Figure 4. Principle component analysis (A) and (orthogonal) partial least squares discriminant analysis (B, C and D) score plots of the datasets obtained by the LC-MS and UHPLC-LTQ Orbitrap MS analysis of B. amyloliquefaciens LZN01 and its growth medium. The quality of the (O)PLS-DA models was evaluated by R2X, R2Y, Q2Y and p values and validated by 200 permutation tests.

Figure 4. Principle component analysis (A) and (orthogonal) partial least squares discriminant analysis (B, C and D) score plots of the datasets obtained by the LC-MS and UHPLC-LTQ Orbitrap MS analysis of B. amyloliquefaciens LZN01 and its growth medium. The quality of the (O)PLS-DA models was evaluated by R2X, R2Y, Q2Y and p values and validated by 200 permutation tests.

Figure 5. Major differential metabolites among experimental groups.

Figure 5. Major differential metabolites among experimental groups.

Figure 6. Effects of myriocin (A), 3-methyl-2-oxovaleric acid (B) and gabapentin (C) on the mycelial growth of Fon.

Figure 6. Effects of myriocin (A), 3-methyl-2-oxovaleric acid (B) and gabapentin (C) on the mycelial growth of Fon.

Figure 7. Quantitative analysis of myriocin in the CFS from LB medium at 72 h (A), CFS from B. amyloliquefaciens LZN01 at 12 h (B) and CFS from B. amyloliquefaciens LZN01 at 72 h (C).

Figure 7. Quantitative analysis of myriocin in the CFS from LB medium at 72 h (A), CFS from B. amyloliquefaciens LZN01 at 12 h (B) and CFS from B. amyloliquefaciens LZN01 at 72 h (C).

Figure 8. The minimal inhibitory concentrations of myriocin against Fon (A) and time-kill curves of myriocin with different concentrations against Fon for 24 h (B). Data are expressed as the means ± SD (n = 3).

Figure 8. The minimal inhibitory concentrations of myriocin against Fon (A) and time-kill curves of myriocin with different concentrations against Fon for 24 h (B). Data are expressed as the means ± SD (n = 3).
Supplemental material

Supplemental Material

Download PDF (516.1 KB)

Supplemental Material

Download PDF (661 KB)