Improving blast resistance of the rice restorer line, Hui 316, by introducing Pi9 or Pi2 with marker-assisted selection

Figures & data

Figure 1. Development of the optimized InDel marker Pi9/2. (A) Pi9/2 primers are designed for targeting the genomic variation of the Pi9 (75-1-127), Pi2 (C101A51) and non-Pi9/Pi2 (Hui 316) alleles in different cultivars. (B) PCR amplification patterns of the InDel marker Pi9/2 differentiating the Pi9, Pi2 and non-Pi9/Pi2 alleles. M: DNA ladder.

Figure 2. Schematic diagram of introgressing Pi9 or Pi2 into Hui 316 with MAS and phenotype selection (PS).

Figure 3. Identification of the homozygous Pi9 or Pi2-introgressed Hui 316 lines by MAS and characterization of Hui 316-Pi9 and Hui 316-Pi2. (A) PCR amplification patterns of marker Pi9/2 identifying 20 homozygous Pi9 or Pi2-introgressed Hui 316 lines. (B) Comparison of the plant and seed morphology of Hui 316, Hui 316-Pi9 and Hui 316-Pi2. Hui 316-Pi9 and Hui 316-Pi2 displayed similar plant and seed traits with Hui 316. Bars, 5 mm; M: DNA ladder.

Table 1. Agronomic traits of Hui 316, Hui 316-Pi9 and Hui 316-Pi2.

Location	Materials	Panicle length (cm)	Plant height (m)	Number of panicle per plant	Weight of 1000-grain (g)	Seed setting rate (%)	Single-plant yield (g)
Sanya	Hui 316	20.60 ± 1.67	0.98 ± 0.04	10.60 ± 1.14	27.16 ± 0.08	80.18 ± 2.54	33.22 ± 1.95
	Hui 316-Pi9	19.58 ± 1.01	0.99 ± 0.01	10.40 ± 0.55	26.75 ± 0.40	86.38 ± 5.32	37.47 ± 6.92
	Hui 316-Pi2	19.35 ± 0.44	0.97 ± 0.02	10.40 ± 1.14	28.24 ± 0.07*	87.15 ± 2.42	37.46 ± 2.04

^* Statistically significant with P-value <0.05.

Table 2. Blast disease incidence of 75-1-127, C101A51, Hui 316, Hui 316-Pi9 and Hui 316-Pi2 seedlings inoculated with 21 Fujian blast fungal isolates and their mixture in greenhouse.

Isolates	75-1-127 (Pi9)	C101A51 (Pi2)	Hui 316	Hui 316-Pi9	Hui 316-Pi2
JY15015	R	R	R	R	R
NH17028	R	R	R	R	R
SM17211	R	R	S	R	R
SH17004	R	R	S	R	R
JL15140	R	R	MS	R	R
NH13093	R	R	R	R	R
NH17267	R	R	R	R	R
SM17192	R	R	S	R	R
JY14027	R	R	R	R	R
NH15039	R	R	S	R	R
SM17212	R	R	S	R	R
NH15040	R	R	R	R	R
NH17026	R	R	MS	R	R
NH17268	R	R	R	R	R
SM17203	R	R	MS	R	R
NH17029	R	R	R	R	R
NJ08022	R	R	R	R	R
SM17207	R	R	S	R	R
SM17191	R	R	S	R	R
NH17025	R	R	R	R	R
NH15092	R	R	R	R	R
Mixture	R	R	R	R	R
Comprehensive	R	R	S	R	R

Table 3. Blast disease incidence of 75-1-127, C101A51, Hui 316, Hui 316-Pi9 and Hui 316-Pi2 grown in the field of Shanghang, Fujian during 2016 and 2017.

Year	75-1-127 (Pi9)	C101A51 (Pi2)	Hui316	Hui 316-Pi9	Hui 316-Pi2
2016 (Shanghang)	R	MR	S	MR	MR
2017 (Shanghang)	MR	MR	HS	MR	R
Comprehensive	R	R	S	R	R

Table 4. Agronomic traits of the hybrid progenies derived from Hui 316, Hui 316-Pi9 and Hui 316-Pi2 crossing with various male sterility lines.

Location	Hybrid	Panicle length (cm)	Plant height (m)	Number of panicle per plant	Weight of 1000- grain (g)	Seed setting rate (%)	Single- plant yield (g)	Brown rice rate (%)	Milled rice rate (%)	Head rice rate (%)	Transparence	Grain length/ breadth
Fuzhou	Huifeng A/Hui 316 Huifeng A/Hui 316-Pi9 Huifeng A/Hui 316-pi2	16.69 ± 1.90 18.50 ± 0.93* 17.34 ± 0.80	1.00 ± 0.02 0.99 ± 0.03 0.98 ± 0.03	12.82 ± 1.64 14.07 ± 0.83 13.07 ± 0.31	27.03 ± 0.38 27.07 ± 0.64 29.20 ± 0.74	83.34 ± 2.10 70.46 ± 5.36* 80.54 ± 3.82	54.33 ± 6.00 42.53 ± 4.46 45.33 ± 6.71	0.78 ± 0.00 0.72 ± 0.02 0.76 ± 0.02	0.36 ± 0.00 0.36 ± 0.00 0.36 ± 0.00	0.29 ± 0.04 0.21 ± 0.01 0.23 ± 0.03	0.66 ± 0.02 0.58 ± 0.09 0.66 ± 0.01	2.62 ± 0.00 2.65 ± 0.01 2.48 ± 0.01*
Sanya	Guang 8A/Hui 316 Guang 8A/Hui 316-Pi9 Guang 8A/Hui 316-pi2	19.02 ± 0.90 22.21 ± 0.64 18.97 ± 0.24	1.03 ± 0.02 1.07 ± 0.03 1.03 ± 0.02	10.81 ± 1.35 13.84 ± 1.96 13.17 ± 2.32	22.49 ± 0.32 23.48 ± 0.60 21.87 ± 0.41	86.05 ± 3.90 80.73 ± 2.50 85.37 ± 5.50	47.87 ± 7.80 52.00 ± 7.20 49.93 ± 8.55	0.73 ± 0.08 0.76 ± 0.00 0.69 ± 0.11	0.35 ± 0.00 0.35 ± 0.00 0.35 ± 0.00	0.23 ± 0.04 0.21 ± 0.06 0.18 ± 0.00	0.58 ± 0.03 0.67 ± 0.00 0.57 ± 0.00	2.86 ± 0.05 3.18 ± 0.05* 2.91 ± 0.02

*Statistically significant with P-value <0.05.