Protective role of H₂S on acute renal damages in urinary-derived sepsis

Figures & data

Table 1. WBC counts at indicated time points in the experimental groups.

	Before surgery	After surgery			P
		24 h	48 h	72 h
Control	10.20 ± 0.14	10.19 ± 0.05	10.16 ± 0.09	10.18 ± 0.07	Control vs. Sham 0.931
Sham	10.16 ± 0.10	10.23 ± 0.08	10.20 ± 0.09	10.19 ± 0.05	Sham vs. Sepsis 0.000
Sepsis	10.17 ± 0.03	17.48 ± 0.47	18.11 ± 0.50	19.22 ± 0.27	Sepsis vs. PAG 0.000
PAG	10.17 ± 0.04	18.55 ± 0.48	20.31 ± 0.89	22.28 ± 0.94	PAG vs. NaHS 0.000
NaHS	10.19 ± 0.04	15.21 ± 0.09	16.14 ± 0.35	15.54 ± 0.29	NaHS vs. Sepsis 0.000
F	0.361	1342.410	720.669	1103.938
P	0.835	0.000	0.000	0.000

Note: F_time =2271.671, F_inter-group =1630.099, F_interaction =453.807; P < 0.01.

Table 2. CRP levels at indicated time points in the experimental groups.

	Before surgery	After surgery			P
		24 h	48 h	72 h
Control	0.35 ± 0.12	0.35 ± 0.14	0.36 ± 0.14	0.36 ± 0.15	Control vs. Sham 0.632
Sham	0.36 ± 0.13	0.47 ± 0.13	0.38 ± 0.10	0.37 ± 0.12	Sham vs. Sepsis 0.000
Sepsis	0.36 ± 0.11	5.68 ± 0.18	6.79 ± 0.20	7.69 ± 0.24	Sepsis vs. PAG 0.000
PAG	0.36 ± 0.16	6.77 ± 0.28	7.82 ± 0.27	9.13 ± 0.35	PAG vs. NaHS 0.000
NaHS	0.35 ± 0.13	3.25 ± 0.18	4.37 ± 0.33	5.54 ± 0.36	NaHS vs. Sepsis 0.000
F	0.016	1870.767	1940.126	1901.871
P	0.999	0.000	0.000	0.000

Note: F_time =5909.168, F_inter-group =2146.009, F_interaction =1111.882; P < 0.01.

Table 3. Peripheral blood Cr levels at indicated time points in the experimental groups.

	Before surgery	After surgery			P
		24 h	48 h	72 h
Control	87.45 ± 4.40	86.89 ± 4.43	86.98 ± 4.81	87.39 ± 4.17	Control vs. Sham 0.989
Sham	86.55 ± 5.22	88.06 ± 4.51	87.09 ± 4.55	86.93 ± 5.41	Sham vs. Sepsis 0.000
Sepsis	87.31 ± 3.88	114.01 ± 5.63	157.03 ± 4.62	187.13 ± 4.67	Sepsis vs. PAG 0.000
PAG	86.66 ± 5.27	131.61 ± 6.98	180.94 ± 4.31	210.19 ± 6.84	PAG vs. NaHS 0.000
NaHS	86.85 ± 4.35	96.84 ± 4.65	117.74 ± 5.07	143.01 ± 3.82	NaHS vs. Sepsis 0.000
F	0.058	102.726	647.116	977.699
P	0.993	0.000	0.000	0.000

Note: F_time =1065.006, F_inter-group =921.089, F_interaction =221.971; P < 0.01.

Table 4. Peripheral blood BUN levels at indicated time points in the experimental groups.

	Before surgery	After surgery			P
		24 h	48 h	72 h
Control	6.08 ± 0.19	6.05 ± 0.17	6.06 ± 0.23	6.09 ± 0.23	Control vs. Sham .660
Sham	6.09 ± 0.25	6.14 ± 0.30	6.12 ± 0.25	6.14 ± 0.18	Sham vs. Sepsis 0.000
Sepsis	6.11 ± 0.28	11.21 ± 0.47	13.35 ± 0.53	15.68 ± 0.48	Sepsis vs. PAG 0.000
PAG	6.10 ± 0.26	13.30 ± 0.37	15.32 ± 0.29	16.95 ± 0.57	PAG vs. NaHS 0.000
NaHS	6.12 ± 0.27	8.14 ± 0.31	10.46 ± 0.42	12.05 ± 0.41	NaHS vs. Sepsis 0.000
F	0.045	718.091	1062.593	1083.063
P	0.996	0.000	0.000	0.000

Note: F_time =2886.252, F_inter-group =1223.684, F_interaction =568.217; P < 0.01.

Figure 1. Histochemical staining of renal tissues. H&E staining was performed to detect the morphology of the renal tissues in the Control (A), Sham (B), Sepsis (C), PAG (D), and NaHS (E) groups, respectively (magnification ×200). Bar = 50 μm.

Figure 2. Expression levels of iNOS in renal tissues. The mRNA (A) and protein (B) expression levels of iNOS were detected with SqRT-PCR and Western blot analysis, respectively. Compared with the Control group, *P < 0.05; compared with the Sepsis group, ^#P < 0.05.

Figure 3. Expression levels of HO-1 in renal tissues. The mRNA (A) and protein (B) expression levels of HO-1 were detected with SqRT-PCR and Western blot analysis, respectively. Compared with the Control group, *P < 0.05; compared with the Sepsis group, ^#P < 0.05.