Cut-off value for KLK3 gene expression from urine sediment to rationalize diagnosis of prostate cancer

Figures & data

Table 1. Number of samples by group and mean RNA concentration.

Group	Number of samples	Mean RNA concentration (ng/µL)
1—prostate carcinoma	45	16.0
2—benign hyperplasia	15	2.4
3—PSA < 1.5 and negative DRE	53	2.9
4—healthy controls	49	3.8
∑	162

Table 2. Primers used in this study.

Primer	Forward (5'–3')	Reverse (5'–3')
KLK3	TCCAATGACGTGTGTGCGCA	CCTTGATCCACTTCCGGTAA
PCA3	CCGAGGGAGACCAGGAAGAT	ATCGATGACCCAAGATGGCG
TMPRSS1-ERG	CGCGGCAGGAAGCCTTA	TCCGTAGGCACACTCAAACAAC
GAPDH	TGAAGGTCGGAGTCAACGGA	CATCGCCCCACTTGATTTTGG

Table 3. Mean ΔCt and 2^−ΔCt values.

Group/Gene	Mean KLK3 (ΔCt/2^−Δ^Ct)	Mean PCA3 (ΔCt/2^−Δ^Ct)	Mean TMPRSS2:ERG (ΔCt/2^−Δ^Ct)
1	0.16/17.09	34.37/0.004	11.92/0.0124
2	3.89/0.214	10.30/0.196	12.9/0.024
3	2.3/0.452	9.37/0.014	5.99/0.0013
4	3.7/0.347	10.17/0.007	11.59/0.0016

Table 4. Analysis of variances for KLK3 gene between groups.

Group	1	2	3	4
1	–	P < 0.05	P < 0.05	P < 0.05
2	P < 0.05	–	P ≥ 0.05	P ≥ 0.05
3	P < 0.05	P ≥ 0.05	–	P < 0.05
4	P < 0.05	P ≥ 0.05	P < 0.05	–

Table 5. Analysis of variances for PCA3 gene between groups.

Group	1	2	3	4
1	–	P < 0.05	P ≥ 0.05	P ≥ 0.05
2	P < 0.05	–	P < 0.05	P < 0.05
3	P ≥ 0.05	P < 0.05	–	P ≥ 0.05
4	P ≥ 0.05	P < 0.05	P ≥ 0.05	–