Identification and characterization of NAC genes in response to abiotic stress conditions in Picea wilsonii using transcriptome sequencing

Figures & data

Table 1. Length distribution of the contigs, transcripts, unigenes from the Trinity de novo assembly.

Length range	Contig	Transcript	Unigene
200–300	19,417,751 (99.53%)	163,556 (50.32%)	144,876 (64.70%)
301–500	46,655 (0.24%)	56,916 (17.51%)	39,152 (17.48%)
501–1000	23,872 (0.12%)	42,324 (13.02%)	19,962 (8.91%)
1001–2000	12,845 (0.07%)	36,053 (11.09%)	11,963 (5.34%)
2000+	8062 (0.04%)	26,196 (8.06%)	7972 (3.56%)
Total number	19,509,185	325,045	223,925
Total length	908,041,432	225,061,179	104,765,699
N50 length	47	1406	572
Mean length	46.54	692.40	467.86

Table 2. Functional annotation of Picea wilsonii transcriptome.

Annotated databases	Unigene	≥300 nt	≥1000 nt
COG	23,348	13,045	6145
GO	38,980	21,465	8792
KEGG	15,416	8915	3932
KOG	38,392	21,713	9542
Pfam	42,586	27,008	13,443
Swiss-Prot	31,787	21,144	10,183
nr	68,973	38,917	16,144
All	73,940	40,159	16,274

Figure 1. Species distribution in the top BLAST hits in the NCBI non-redundant (Nr) database.

Figure 2. Functional annotation of the assembled sequences based on gene ontology (GO)categories.

Figure 3. Classification of unigenes based on the Clusters of Orthologous Groups (COG) database.

Figure 4. Pathway assignment of the unigenes based on the Kyoto Encyclopedia for Genes and Genomes (KEGG) database.

Figure 5. Expression of 13 NAC genes under salt and drought treatments.

Figure 6. Phylogenetic analysis of Picea wilsonii and Arabidopsis thaliana NAC genes.

Figure 7. Expression profiles of 13 NAC genes under low temperature and hydrogen peroxide (H₂O₂) treatments.

Figure 8. Expression profiles of 13 NAC genes under phytohormone treatments.

Figure 9. Expression profiles of 13 NAC genes under the wounding treatment.