SRAP markers for genetic diversity assessment of lavender (Lavandula angustifolia mill.) varieties and breeding lines

Figures & data

Table 1. Sequences of SRAP primers used in the analysis.

Forward primer (5′–3′)		Reverse primer (5′–3′)
ME1	tgagtccaaaccggaaa	EM1	gactgcgtacgaattaaa
ME2	tgagtccaaaccggagc	EM2	gactgcgtacgaatttgc
ME3	tgagtccaaaccggtaa	EM3	gactgcgtacgaattatt
ME4	tgagtccaaaccggacc	EM4	gactgcgtacgaattata
ME5	tgagtccaaaccggaag	EM5	gactgcgtacgaattcaa
ME6	tgagtccaaaccggaga	EM6	gactgcgtacgaattttt
ME7	tgagtccaaaccggtcc	EM7	gactgcgtacgaattagc
ME8	tgagtccaaaccggctg	EM8	gactgcgtacgaattcac
ME9	tgagtccaaaccggttc	EM9	gactgcgtacgaattaat
ME10	tgagtccaaaccggtga	EM10	gactgcgtacgaatt tca

Table 2. Summary of SRAP fragments data following SRAP analysis of all studied genotypes.

Primer pair	SRAP fragments					Primer pair	SRAP fragments
	T	TP	PPF (%)	APFG	PIC		T	TP	PPF (%)	APFG	PIC
ЕМ1_МЕ1	93	78	84%	15.6 ± 6.3	0.26	ЕМ6_МЕ1	48	34	71%	10.3 ± 3.2	0.30
ЕМ1_МЕ2	70	42	60%	14.2 ± 3.7	0.29	ЕМ6_МЕ4	173	151	87%	26.4 ± 4.3	0.25
ЕМ1_МЕ3	135	115	85%	26.9 ± 6.2	0.25	ЕМ6_МЕ5	78	59	76%	46.6 ± 7.5	0.33
ЕМ1_МЕ6	115	91	79%	27.7 ± 6.5	0.30	ЕМ6_МЕ6	134	109	81%	23.8 ± 4.6	0.26
ЕМ1_МЕ10	98	71	72%	15.9 ± 9.9	0.25	ЕМ6_МЕ10	201	180	90%	44.0 ± 10.8	0.27
ЕМ2_МЕ3	220	156	71%	50.2 ± 11.3	0.28	ЕМ7_МЕ5	101	74	73%	21.5 ± 6.0	0.25
ЕМ2_МЕ7	164	108	66%	20.6 ± 8.8	0.22	ЕМ7_МЕ9	49	14	29%	6.3 ± 1.6	0.39
ЕМ2_МЕ9	136	104	76%	44.4 ± 5.8	0.26	ЕМ7_МЕ10	205	182	89%	38.1 ± 9.1	0.23
ЕМ2_МЕ10	209	185	89%	52.6 ± 13.3	0.26	ЕМ8_МЕ1	156	127	81%	39.5 ± 7.3	0.24
ЕМ3_МЕ1	164	138	84%	34.1 ± 7.9	0.27	ЕМ8_МЕ2	104	87	84%	31.8 ± 4.2	0.22
ЕМ3_МЕ3	112	90	80%	18.9 ± 4.7	0.25	ЕМ8_МЕ6	38	29	76%	13.5 ± 3.1	0.31
ЕМ3_МЕ6	62	36	58%	8.5 ± 4.8	0.26	EM8_ME7	99	82	83%	26.5 ± 6.0	0.27
ЕМ3_МЕ10	109	93	85%	18.6 ± 4.5	0.24	ЕМ8_МЕ8	94	63	67%	20.4 ± 4.6	0.31
ЕМ4_МЕ1	213	207	97%	47.6 ± 15.5	0.26	ЕМ8_МЕ10	93	77	83%	20.9 ± 5.6	0.25
ЕМ4_МЕ3	154	119	77%	26.1 ± 9.4	0.27	ЕМ9_МЕ2	107	92	86%	23.6 ± 6.8	0.29
ЕМ4_МЕ5	76	47	62%	13.1 ± 2.4	0.25	ЕМ9_МЕ4	86	57	66%	14.6 ± 3.4	0.26
ЕМ4_МЕ6	99	78	79%	21.8 ± 5.1	0.25	ЕМ9_МЕ5	151	117	77%	29.6 ± 7.6	0.26
ЕМ4_МЕ10	82	41	50%	11.4 ± 2.1	0.26	ЕМ9_МЕ9	110	84	76%	21.3 ± 5.6	0.25
EM5_ME1	95	64	67%	26.4 ± 2.5	0.30	ЕМ9_МЕ10	165	126	76%	28.3 ± 7.2	0.24
ЕМ5_МЕ2	88	40	45%	18.0 ± 2.7	0.28	ЕМ10_МЕ1	87	66	76%	23.6 ± 11.1	0.32
ЕМ5_МЕ3	130	114	88%	25.4 ± 6.0	0.25	ЕМ10_МЕ5	107	82	77%	25.3 ± 5.6	0.26
ЕМ5_МЕ4	184	144	78%	36.6 ± 7.2	0.26	ЕМ10_МЕ8	151	134	89%	29.6 ± 5.3	0.24
ЕМ5_МЕ5	87	40	46%	16.6 ± 2.8	0.29	ЕМ10_МЕ9	149	126	85%	15.4 ± 4.2	0.24
ЕМ5_МЕ6	76	47	62%	15.9 ± 4.6	0.30	ЕМ10_МЕ10	117	92	79%	27.4 ± 5.7	0.27
ЕМ5_МЕ7	82	46	56%	20.5 ± 9.8	0.33	Total	6088	4697	77.2%
ЕМ5_МЕ8	100	58	58%	19.9 ± 4.0	0.29	Average	119.4 ± 44.9	92.1 ± 44.5	74.3% ± 13.3%	25.5 ± 12.8	0.27 ± 0.03
ЕМ5_МЕ10	132	101	77%	27.4 ± 7.4	0.27

T, total number of different SRAP fragments observed for the tested primer pair in all analyzed genotypes; TP, total number of different polymorphic SRAP fragments observed for the tested primer pair in all analyzed genotypes; PPF, percent of the polymorphic fragments for the tested primer pair; APFG, average number of polymorphic fragments for the tested primer pair observed for one genotype; PIC, polymorphism information content.

Figure 1. Neighbour-joining phylogenetic tree constructed from SRAP data following analysis of Bulgarian industrially cultivated lavender essential oil varieties and breeding lines, and foreign lavender cultivars. The names of the analyzed varieties and breeding lines are shown on the right. The cluster formed by foreign varieties is designated with the abbreviation ‘FV’.