Association between quantitative analyses of periodontal pathogens and the depth of periodontal pockets

Figures & data

Table 1. Primers for Hum P, Bact16S, Aa, Pg, Pi, Td and Tf.

Gene	Primers	Sequence (5'------3')	NCBI Reference Sequence	bp
Hum P	Forward	GATTTGGACCTGCGAGCG	NM_001104546.2	64
	Reverse	GAGCGGCTGTCTCCACAAGT
	Probe	TTCTGACCTGAAGGCTCTGCGCG
Bact16S	Forward	TCCTACGGGAGGCAGGCAGT	GenBank: LC176649.1	470
	Reverse	GGACTACCAGGGTATCTAATCCTGTT
	Probe	CGTRTTACCGCGGCTGCTGGCAC
Aa	Forward	ACCTTACCTACTCTTGACATCCGAA	GenBank: MK270928.1	77
	Reverse	GCAGCACCTGTCTCAAAGC
	Probe	AGAACTCAGAGATGGGTTTGTGCCTTAG
Pg	Forward	GCAGCTTGCCATACTGCG	GenBank: KX108929.1	136
	Reverse	CTTTCGCTGTGGAAGCTTGA
	Probe	CTCCTAGTAATCATCGTTTACTGCGTGGACTA
Pi	Forward	CCACATATGGCATCTGACGTG	GenBank: CP024732.1	233
	Reverse	TCAATCCTGCACGCTACTTGG
	Probe	ACCAAAGATTCATCGGTGGAGGATGGG
Td	Forward	CCGAATGTGCTCATTTACATAAAGGT	GenBank: KT192147.1	122
	Reverse	GATACCCATCGTTGCCTTGGT
	Probe	GATGGGCCCGCGTCCCATTAGCT
Tf	Forward	CCGGGATTGAAATGTAGACG	GenBank: AP013044.1	155
	Reverse	CTTTACCTGTTAGCAACTGACAGTCA
	Probe	CGGAGAGTGAGAGCTTTCTTCCCTTC

Figure 1. Relative expression of Pg (A), Td (B), Tf (C), Pi (D), Aa (E) and total bacteria (F) in the periodontium of patients.

Note: Data are the mean ± SEM (healthy controls, n = 426; moderate periodontitis, n = 602; severe periodontitis, n = 36). **P < 0.01 vs. healthy controls, ^##P < 0.01 vs. moderate-periodontitis group.

Figure 2. Correlation analyses among different periodontal pathogens (A–C) or between periodontal pathogens and probing depth (D–E). n = 1164, **p < 0.01.

Table 2. Correlation coefficient for different periodontal pathogens or probing depth.

	Probing depth	16SrDNA-ROX	Pg-CYS5	Td-ROX	Tf-FAM	Aa-FAM
16SrDNA-ROX	−0.028
Pg-CY5	0.532**	−0.011
Td-ROX	0.358**	−0.045	0.543**
Tf-FAM	0.481**	−0.052	0.631**	0.693**
Aa-FAM	0.025	−0.038	0.014	0.024	0.014
Pi-HEX	0.158**	0.103**	0.169**	0.161**	0.115**	−0.004

n = 1164, **p < 0.01.

Figure 3. ROC curves were constructed to evaluate the diagnostic potential of periodontal pathogens for periodontitis (A–D).