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Research Article

De novo assembly and characterization of the transcriptome and development of microsatellite markers in a Chinese endemic Euphorbia kansui

, , , , , & show all
Pages 562-574 | Received 15 Jan 2020, Accepted 22 Jun 2020, Published online: 08 Jul 2020

Figures & data

Table 1. Summary of transcriptome sequencing dataset.

Figure 1. Assembly length distribution of contigs and unigenes in Euphorbia kansui.

Figure 1. Assembly length distribution of contigs and unigenes in Euphorbia kansui.

Figure 2. Number of unigenes annonated in protein databases by BLASTx with E-value threshold of 10−5. The numbers in the circles indicate the number of unigenes annotated by single or multiple databases.

Figure 2. Number of unigenes annonated in protein databases by BLASTx with E-value threshold of 10−5. The numbers in the circles indicate the number of unigenes annotated by single or multiple databases.

Figure 3. Gene ontology classification of assembled unigenes of Euphorbia kansui. In total 15,506 unigenes were categorized into three main categories: biological process, cellular component and molecular function.

Figure 3. Gene ontology classification of assembled unigenes of Euphorbia kansui. In total 15,506 unigenes were categorized into three main categories: biological process, cellular component and molecular function.

Figure 4. Cluster of Orthologous Groups (COG) function classification of Euphorbia kansui unigenes. A total of 13,528 unigenes were annotated and classified into 25 different classifications.

Figure 4. Cluster of Orthologous Groups (COG) function classification of Euphorbia kansui unigenes. A total of 13,528 unigenes were annotated and classified into 25 different classifications.

Figure 5. Terpenoid backbone and diterpene biosynthesis pathways. Terpenoid backbone (isopentenyl diphosphate) is synthesized from two distinct pathways, methylerythritol phosphate (MEP/DXP) pathway and mevalonate (MVA) pathway. The enzymes participating in each step of these pathways are listed in .

Figure 5. Terpenoid backbone and diterpene biosynthesis pathways. Terpenoid backbone (isopentenyl diphosphate) is synthesized from two distinct pathways, methylerythritol phosphate (MEP/DXP) pathway and mevalonate (MVA) pathway. The enzymes participating in each step of these pathways are listed in Table 2.

Table 2. Identified unigenes of terpenoid backbone and diterpene biosynthesis.

Figure 6. Distribution of simple sequence repeats (SSR) in Euphorbia kansui: distribution of different repeat types (a); number of SSRs per different tandem repeat motifs (b).

Figure 6. Distribution of simple sequence repeats (SSR) in Euphorbia kansui: distribution of different repeat types (a); number of SSRs per different tandem repeat motifs (b).

Figure 7. Frequency distribution of main SSR repeat motifs.

Figure 7. Frequency distribution of main SSR repeat motifs.

Table 3. Summary of SSR search results.

Table 4. Characterization of 23 polymonophic SSR markers in Euphorbia kansui.

Supplemental material

Supplemental Material

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