Cloning, molecular properties and differential expression analysis of the isopentenyl diphosphate isomerase gene in Sanghuangporus baumii

Figures & data

Table 1. Primers used in the present study.

Primer name and annotation	Primer sequence	Predicted band (bp)
SbIDI-F1 (RT-PCR)	5ˊ-TATCGGATCCGAATTCATGTCTGCTACTGCTACTACTAC-3ˊ	824
SbIDI-R1	5ˊ-GTGCGGCCGCAAGCTTTCAAACCATCTTCACGACCTTAC-3ˊ
pET32a-F (RT-PCR)	5ˊ-TAATACGACTCACTATAGGG-3ˊ	712
pET32a-R	5ˊ-GCTAGTTATTGCTCAGCGG-3ˊ
SbIDI-F2 (qRT-PCR)	5ˊ-GCTGCTGCGGCGTTTGCTACTAT-3ˊ	239
SbIDI-R2	5ˊ-GCTGCTGCGGCGTTTGCTACTAT-3ˊ
α-tubulin-F (qRT-PCR)	5ˊ-CCAGCAAGCGTTACCGATT-3ˊ	129
α-tubulin-R	5ˊ-TCCACGACGTCCATCGTTC-3ˊ
β-tubulin-F (qRT-PCR)	5ˊ-GCTGAATATCGTTCGTGCCC-3ˊ	170
β-tubulin-R	5ˊ-ATCCGCCTTCCTCCTTACAGT-3ˊ

Figure 1. cDNA sequence of SbIDI gene from S. baumii and its predicted amino acid sequence.

Figure 2. Three-dimensional structure prediction of the SbIDI protein.

Figure 3. Phylogenetic analysis of IDI protein of S. baumii and IDI protein of other species.

Figure 4. Triterpenoids content of S. baumii at different developmental stages (p < 0.05).

Figure 5. The transcript level of the SbIDI gene at different developmental stages of S. baumii (p < 0.05).

Figure 6. Prokaryotic expression of SbIDI gene cDNA in E. coli BL21(DE3) with IPTG induction. Lane 1 is the blank control, E. coli BL21(DE3) harbouring pET-32a vector without IPTG induction; lanes 2–7 E. coli BL21(DE3) harbouring vector pET-32a-SbIDI induced by IPTG (1 mmol/L) at 0, 2, 4, 6, 8, and 10 h, respectively; lane M is the protein marker (Solarbio, Beijing, China).