Initial safety evaluation of Enterococcus faecium LBB.E81

Figures & data

Table 1. Minimal inhibition concentrations (MIC) of a set of antibiotic substances compared to resistance threshold values defined by EUCAST [19].

Tested antibiotic	Range tested (mg/L)	MIC (mg/L)
		Threshold	E. durans LMG 10746^T	E.faecalis LMG 7937^T	E. faecium LMG 11423^T	E. faecium LBB.E81
Tigecycline	0.015 − 0.5	–	0.12	0.25	0.12	0.12
Tetracycline	1 − 32	–	< 1	< 1	< 1	< 1
Chloramphenicol	2 − 32	–	8	8	8	8
Daptomycin	0.25 − 16	–	1	2	2	1
Streptomycin	512 − 2048	> 512	< 512	< 512	< 512	< 512
Tylosine tartarate	0.25 − 32	–	2	2	2	2
Quinupristin/dalfopristin	0.5 − 32	> 4	< 0.5	2	< 0.5	< 0.5
Linezolid	1 − 8	> 4	2	2	4	4
Nitrofurantoin	2 − 64	> 64	> 64	16	> 64	> 64
Penicillin	0.25 − 16	> 8	4	4	2	0.25
Kanamycin	128 − 1024	–	< 128	< 128	< 128	< 128
Erythromycin	0.25 − 8	–	< 0.25	1	4	8
Ciprofloxacin	0.12 − 4	> 4	0.5	2	> 4	4
Vancomycin	0.25 − 4	> 4	1	1	1	0.5
Lincomycin	1 − 8	–	< 1	> 8	< 1	< 1
Gentamycin	128 − 1024	> 128	< 128	< 128	< 128	< 128

Note: Numbers in boldface indicate MIC values that exceed the threshold level set by EUCAST for antibiotic resistance of enterococci.

Figure 1. Alignment of the predicted amino-acid N-terminal sequence, encoded by the collagen-binding factor gene acm in E. faecium LBB.E81 (missing the N-terminally located signal sequence necessary for export of the protein) and the collagen binding clinical isolate E. faecium TX2555 [44; Acc. No. AY135217).

EUCAST, The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 10.0, 2020. http://www.eucast.org.

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Nallapareddy SR, Weinstock GM, Murray BE. Clinical isolates of Enterococcus faecium exhibit strain-specific collagen binding mediated by Acm, a new member of the MSCRAMM family . Mol Microbiol. 2003;47(6):1733–1747.

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Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article.