Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu

Figures & data

Table 1. Effects of purified avenin-like proteins on the mixograph parameters.

Sample	MT (s)	PR (AU)	RBD (AU)
Base flour (Gaoyuan 448)	118 ± 4.15 a	510 ± 18.45 a	16 ± 1.06 a
Base flour, DTT, KIO3	120 ± 7.05 a	521 ± 13.34 a	15 ± 1.47 a
Base flour, DTT, KIO3,TuALPb7A-G	139 ± 3.12 b	581 ± 28.01 b	10 ± 0.63 b
Base flour, DTT, KIO3,TuALPb7A-O	138 ± 5.04 b	570 ± 38.14 b	10 ± 0.39 b
Base flour, DTT, KIO3,TuALPb7A-K	141 ± 2.16 b	601 ± 26.59 b	8 ± 0.73 b
Base flour, DTT, KIO3,TuALPb7A-C	138 ± 4.38 b	588 ± 19.63 b	9 ± 0.56 b
Base flour, DTT, KIO3,TaALPb-7A1	142 ± 2.86 b	611 ± 20.47 b	8 ± 1.13 b

MT, mixing time; PR, peak dough resistance; RBD, resistance at breakdown. Data are mean values of duplicate experiments ± standard deviation. Means with different small letters are significantly different at p < 0.05 level.

Figure 1 Alignment of the deduced amino acid sequences of avenin-like b genes. Inverted triangles indicate the position of the cysteines. Signal: signal peptide; N-term: N-terminus; Rep-region L: Repetitive region in the left; Rep-region R: Repetitive region in the right; C-term: C-terminus.

Figure 2. Cys residues position comparison between T. urartu and Ae. tauschii.

Figure 3. Neighbor-joining tree based on complete nucleotide sequences of avenin-like b genes.

Figure 4. Identification of expression of avenin-like b protein and purified protein by SDS-PAGE. Lane M: Marker (116.0/66.2/45.0/35.0/25.0 kDa, Shanghai Dingjie Biotechnology Co., LTD); Lane 1: expression of supernatant protein induced by IPTG; Lane 2: expression of recombinant protein induced by IPTG; Lane 3: supernatant protein after purification; Lane 4: recombinant protein after purification; Lane 5: Renatured avenin-like b protein before purification; Lane 6: Renatured avenin-like b protein after purification.

Data availability statement

The data that support this study are available from the corresponding authors upon reasonable request.