Expression analysis of miRNAs and their targets related to salt stress in Solanum lycopersicum H-2274

Figures & data

Table 1. Primer sequences of the stem loop primers.

Primers	Sequences
ath-miR398a-5p	5’GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGTGTT3’
ath-miR845a	5’GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCATCAA3’
ath-miR170-5p	5′GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCTGAG3’
sly-miR395a	5′GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGAGTT3′
sly-miR171a	5’GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGATATT3’
sly-miR156a	5’GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTGCTC3’

Table 2. Primer sequences of the selected miRNAs used in qPCR analysis.

Primers	Sequences
ath-miR398a-5p	5’GCGGCGGAAGGAGTGGCATGTG3’
ath-miR845a	5’GCGGCGGCGGCTCTGATACC3’
ath-miR170-5p	5′GCGGCGGTATTGGCCTGGTTCAC3’
sly-miR395a	5′GCGGCGGCTGAAGTGTTTGGGG3′
sly-miR171a	5’GCGGCGGTGATTGAGCCGTGCC3’
sly-miR156a	5’GCGGCGGTTGACAGAAGATAGAG3’
Universal Reverse	5’ATCCAGTGCAGGGTCCGAGG3’

Table 3. Primer sequences of the selected miRNA targets used in qPCR analysis.

Primers	miRNA Acc.	Target Acc.	Sequences
Target 1.1	ath-miR398a-5p	>Solyc02g023980.2.1	5′-TGCAGCTCAGGCATTGATTG-3′
			5′-AGCCCAAGATGGATCTGCATAG-3′
Target 1.2	ath-miR398a-5p	>Solyc01g080010.2.1	5′-ACCAGCAGTGCCATCAATTG-3′
			5′-ACACCACCATCCAAAACTCC-3′
Target 2.1	sly-miR171a	>Solyc08g078800.1.1	5′-TGCCTCGTCATCAATTGCAG-3′
			5′-TGCGCGTTTGAGAATTGTCC-3′
Target 2.2	sly-miR171a	>Solyc01g090950.2.1	5′-TGGGGACAAAGCCAAAGATG-3′
			5′-ATCGCTTGTTGTTGCTGCTG-3′
Target 2.3	sly-miR171a	>Solyc02g085600.1.1	5′-TTGGACTAAGCCAGTTTGCC-3′
			5′-TGTCATGCCAGCAAAGCAAC-3′
Target 3.1	sly-miR156a	>Solyc02g077920.2.1	5′-TTTGGCAGGTCACAATGAGC-3′
			5′-TTTGGCTGCCATTGATGCTG-3′
Target 3.2	sly-miR156a	>Solyc10g078700.1.1	5′-TTTGGAAATGCGGGTGTTGG-3′
			5′-AACTTGGCACCTAGGTGGATG-3′
Target 3.3	sly-miR156a	>Solyc04g045560.2.1	5′-TGACGCATGCAATTCCTCAG-3′
			5′-TGTGACCATTCGCAACCAAC-3′
Target 3.4	sly-miR156a	>Solyc05g015510.2.1	5′-AGAACTGGCAGCACAAATGG-3′
			5′-AGATGCGTTACTGTGTGCAC-3′
Target 3.5	sly-miR156a	>Solyc05g012040.2.1	5′-GGTTTTGTCAGCAGTGTAGCAG-3′
			5′-TTTGCGACAGCTGCGTTTAC-3′
Target 4.1	sly-miR395a	>Solyc04g054730.2.1	5′-TGCCGGTGTTTTTCAAGCTG-3′
			5′-AGCAGCACCAGCCATAAAAC-3′

Figure 1. Morphological effects of NaCl application on H-2274 tomato plants: control (not treated) plants (a) and plants treated with 100 mmol/L NaCl for 7 days (b).

Figure 2. Graphical representation of the changes in the expression levels of selected miRNAs and their targets in H-2274 tomato plants exposed to salt stress. Expression profiles of miRNAs in root tissue (A) and stem tissue (B); and expression profiles of miRNA targets in root tissue (C) and stem tissue (D).

Data availability statement

All data that support this study are available from the corresponding author upon reasonable request.