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Articles

Genomics-guided isolation and identification of active secondary metabolites of Bacillus velezensis BA-26

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Pages 895-904 | Received 14 Dec 2020, Accepted 20 May 2021, Published online: 06 Jul 2021

Figures & data

Figure 1. Genome circle map (A) and COG function annotation (B) of B. velezensis BA-26. (A) The circle map shows seven kinds of information from the outside to the inside: circle 1 is genome location information, circle 2 is GC content information, circle 3 is coding gene on positive chain (red label), circle 4 is coding gene on negative chain (green label), circle 5 is ncRNA information on the positive chain (blue mark), circle 6 is ncRNA information on the negative chain (purple label), and circle 7 marks the information of long fragment repeats (≥ 100bp) in the genome (orange tagging). (B) Functional classification of COG genes of strain BA-26.

Figure 1. Genome circle map (A) and COG function annotation (B) of B. velezensis BA-26. (A) The circle map shows seven kinds of information from the outside to the inside: circle 1 is genome location information, circle 2 is GC content information, circle 3 is coding gene on positive chain (red label), circle 4 is coding gene on negative chain (green label), circle 5 is ncRNA information on the positive chain (blue mark), circle 6 is ncRNA information on the negative chain (purple label), and circle 7 marks the information of long fragment repeats (≥ 100 bp) in the genome (orange tagging). (B) Functional classification of COG genes of strain BA-26.

Table 1. Genomic characteristics of B. velezensis BA-26.

Figure 2. Functional annotation of secondary metabolite-related genes in B. velezensis BA-26 using KEGG metabolic pathway analysis.

Figure 2. Functional annotation of secondary metabolite-related genes in B. velezensis BA-26 using KEGG metabolic pathway analysis.

Figure 3. GO functional classification of B. velezensis BA-26 genes.

Figure 3. GO functional classification of B. velezensis BA-26 genes.

Figure 4. Gene distribution of CAZy family in the B. velezensis BA-26 genome.

Figure 4. Gene distribution of CAZy family in the B. velezensis BA-26 genome.

Figure 5. LC-MS/MS analysis of iturin and fengycin A. C15 iturin A (A) and fengycin A (B–F) series compounds were identified by mass spectrometry and both showed strong antifungal activity against B. cinerea.

Figure 5. LC-MS/MS analysis of iturin and fengycin A. C15 iturin A (A) and fengycin A (B–F) series compounds were identified by mass spectrometry and both showed strong antifungal activity against B. cinerea.
Supplemental material

Supplemental Material

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Data availability

All data that support the findings from this study are available from the corresponding author (…) upon reasonable request.