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Articles

Ethanolic extract of Turkish bee pollen and propolis: phenolic composition, antiradical, antiproliferative and antibacterial activities

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Pages 45-56 | Received 06 Sep 2021, Accepted 17 Feb 2022, Published online: 21 Mar 2022

Figures & data

Table 1. Gradient program of HPLC.

Table 2. HPLC conditions and settings.

Figure 1. HPLC analysis. (A) Standard chromatogram. 1: Gallic Acid, 2: Protocatechic Acid, 3: Catechin, 4: P-Hydroxy Benzoic Acid, 5: Chlorogenic Acid, 6: Caffeic Acid, 7: Epicatechin, 8: Syringic Acid, 9: Vanilin, 10: P-Coumaric Acid, 11: Ferulic Acid, 12: Sinapinic Acid, 13: Benzoic Acid, 14: O-Coumaric Acid, 15: Rutin, 16: Hesperidin, 17: Rosmarinic Acid, 18: Eriodictiol, 19: Cinnamic Acid, 20: Quercetin, 21: Luteolin, 22: Kaempferol, 23: Apigenin. (B) Chromatogram of the bee pollen ethanolic extract. (C) Chromatogram of the propolis ethanolic extract.

Figure 1. HPLC analysis. (A) Standard chromatogram. 1: Gallic Acid, 2: Protocatechic Acid, 3: Catechin, 4: P-Hydroxy Benzoic Acid, 5: Chlorogenic Acid, 6: Caffeic Acid, 7: Epicatechin, 8: Syringic Acid, 9: Vanilin, 10: P-Coumaric Acid, 11: Ferulic Acid, 12: Sinapinic Acid, 13: Benzoic Acid, 14: O-Coumaric Acid, 15: Rutin, 16: Hesperidin, 17: Rosmarinic Acid, 18: Eriodictiol, 19: Cinnamic Acid, 20: Quercetin, 21: Luteolin, 22: Kaempferol, 23: Apigenin. (B) Chromatogram of the bee pollen ethanolic extract. (C) Chromatogram of the propolis ethanolic extract.

Table 3. Phenolic compounds of bee pollen and propolis ethanolic extracts.

Figure 2. Scavenging activity of propolis and pollen extracts against DPPH radicals at different concentrations.

Figure 2. Scavenging activity of propolis and pollen extracts against DPPH radicals at different concentrations.

Figure 3. Effects of pollen and propolis extracts on viability of Myeloma cells. MTT assay.

Values are means from 3 independent experiments, with standard deviation (±S.D.)

Figure 3. Effects of pollen and propolis extracts on viability of Myeloma cells. MTT assay.Values are means from 3 independent experiments, with standard deviation (±S.D.)

Table 4. Antimicrobial activity of pollen and propolis extracts against selected pathogens using agar well diffusion method (mm).

Table 5. Minimum inhibitory and bactericidal concentrations of bee pollen and propolis extracts against selected pathogens (mg/mL).

Figure 4. MIC values of the extracts for all tested bacteria.

Figure 4. MIC values of the extracts for all tested bacteria.

Data availability statement

The data that support the findings reported in this study are available from the corresponding author upon reasonable request.