Induction of gene silencing of NIb gene region of Potato virus Y by dsRNAs and siRNAs and reduction of infection in potato plants cultivar Djeli

Figures & data

Figure 1. dsRNAs, specific for the NIb genetic region of PVY visualized by gel electrophoresis. L, Ladder (GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific Inc.); S, Sample.

Figure 2. DAS-ELISA of potato plants after treatment with PVY^N/NTN-NIb-specific dsRNAs and siRNAs after infection.

Note: dsRNAs-NIb, Potato plants treated with dsRNAs specific for the HIb-region of PVY, and infected; siRNAs-NIb, Potato plants treated with siRNAs specific for the HIb-region of PVY, and infected; K − dsRNA, Potato plants treated with dsRNA specific for the S-segment of Phi6, and infected; K − siRNA, Potato plants treated with siRNA specific for the S-segment of Phi6, and infected; K − healthy, non-treated healthy potato plants; K + infected, potato plants inoculated with virus only; K − water, non-treated healthy potato plants inoculated with water only; K − kit, negative control of the ELISA kit; K + kit, positive control of the ELISA kit; K − buffer, negative buffer control of the ELISA kit.