Functional characterization of Medicago truncatula GRAS7, a member of the GRAS family transcription factors, in response to abiotic stress

Figures & data

Figure 1. Relative transcript level in T₁ transgenic lines with modified expression MtGRAS7^OE (A) and MtGRAS7^RNAi (B) compared to WT plants. Data represent the mean values ± SEM (n = 3). Asterisks indicate statistically significant differences compared to the control: **p < 0.01; ***p < 0.001.

Figure 2. Phenotype comparison of T₁ transgenic lines with modified expression MtGRAS7^OE and MtGRAS7^RNAi compared to WT plants. Shape of root in WT, GRAS-OE and GRAS-RNAi (A); distribution of nodules in WT, GRAS-OE and GRAS-RNAi (B); shape of flowers and pods in WT, GRAS-OE and GRAS-RNAi (C).

Figure 3. Plant architecture of T₁ transgenic lines with modified expression MtGRAS7^OE and MtGRAS7^RNAi compared to WT plants.

Figure 4. Relative transcript level of MtGRAS7 in T₁ transgenic lines with modified expression of MtGRAS7^OE and MtGRAS7^RNAi compared to WT plants after stress induced by 350 mmol/L mannitol treatment for 0, 24, 48 and 72 h. Leaves (A); Nodules (B). Data represent the mean level of expression of three independent transgenic lines and three control plants ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.

Figure 5. Relative transcript level of MtGRAS7 in T₁ transgenic lines with modified expression MtGRAS7^OE and MtGRAS7^RNAi compared to WT plants after stress induced by 150 mmol/L NaCl treatment for 0, 24, 48 and 72 h. Leaves (A); Nodules (B). Data represent the mean level of expression of three independent transgenic lines and three control plants ± SEM. **p < 0.01; ***p < 0.001.

Figure 6. Seed production of MtGRAS7^OE transgenic lines with modified expression and control 2HA-Wt after application of drought and salinity stress.

Figure 7. Relative transcript level of MtGRAS7 in T₁ transgenic lines with modified expression MtGRAS7^OE and MtGRAS7^RNAi compared to WT plants after stress induced by cold (4 °C) for 0 h, 72 h and 7 days; R, recovery period of one week. Data represent the mean level of expression of three independent transgenic lines and three control plants ± SEM. *p < 0.05; ***p < 0.001.

Data availability

All data that support the findings reported in this study are available from the corresponding author upon reasonable request.