Microbial functional diversity in rhizosphere and non-rhizosphere soil of different dominant species in a vegetation concrete slope

Figures & data

Table 1. Physical and chemical properties of soil.

Sampling plot	Organic matter OM(g/kg)	Total nitrogen TN(g/kg)	Available nitrogen AN(mg/kg)	Total phosphorus TP(g/kg)	Available phosphorus AP(mg/kg)	Total potassium TK(g/kg)	Available potassium AK(mg/kg)	Potential of hydrogen pH	Moisture content WC(%)
GT-R	38.52 ± 1.23Aa	1.83 ± 0.03Ab	39.42 ± 1.06Ba	1.53 ± 0.00Aa	11.99 ± 0.80Bb	10.28 ± 0.12Bc	198.26 ± 0.57Bc	5.41 ± 0.12Bc	30.77
GT-S	15.07 ± 0.51Bb	1.03 ± 0.09Bc	17.58 ± 0.78Aa	1.33 ± 0.06Ba	48.1 ± 3.28Aa	21.16 ± 0.41Aa	576.63 ± 54.85Aa	6.15 ± 0.15Ab	30.43
JC-R	27.56 ± 0.76Ab	1.64 ± 0.04Ac	16.55 ± 0.63Ab	1.41 ± 0.04Ab	70.23 ± 2.22Aa	21.07 ± 0.62Aa	564.76 ± 2.29Aa	6.37 ± 0.08Ab	27.24
JC-S	20.35 ± 0.59Ba	1.27 ± 0.06Bb	14.54 ± 0.6Ba	1.26 ± 0.06Ba	49.82 ± 7.81Ba	20.81 ± 0.56Aab	556.84 ± 2.28Bb	6.21 ± 0.13Ab	23.93
SH-R	22.41 ± 0.26Ac	2.19 ± 0.07Aa	17.47 ± 0.61Ab	1.48 ± 0.05Aab	57.31 ± 1.01Ba	19.14 ± 0.35Bb	307.12 ± 4.00Ab	6.88 ± 0.12Aa	28.93
SH-S	15.53 ± 0.14Bb	1.67 ± 0.05Ba	14.87 ± 1.89Aa	1.38 ± 0.12Aa	50.58 ± 0.80Aa	20.10 ± 0.21Ac	230.92 ± 1.14Bc	7.05 ± 0.20Aa	26.10

Note: PA, Pennisetum alopecuroides; AH, Arthraxon hispidus and PL, Pueraria lobata; R, rhizosphere soil; S, non-rhizosphere soil. Different capital letters indicate significant differences between rhizosphere and non-rhizosphere soils (p<0.05), and different lowercase letters indicate significant differences between different plants in the same type of soil (p<0.05). (The same below).

Table 2. Microbial biomass of soil.

Sample plot	Microbial biomass carbon MBC(mg/kg)	Microbial biomass nitrogen MBN(mg/kg)	Microbial biomass phosphorus MBP(mg/kg)
PL-R	76.25 ± 6.06 Bc	5.82 ± 0.02Ba	10.57 ± 0.34Bb
PL-S	452.28 ± 6.02 Aa	20.98 ± 0.01Aa	12.84 ± 0.62Ac
AH-R	232.65 ± 6.14Bb	5.15 ± 0.06Bb	16.43 ± 0.28Ba
AH-S	290.70 ± 5.17Ab	14.17 ± 0.03Ab	24.99 ± 0.57Aa
PA-R	259.26 ± 0.71Ba	5.87 ± 0.01Ba	15.10 ± 1.48Aa
PA-S	297.34 ± 2.47Ab	12.83 ± 0.97Ab	17.28 ± 0.59Ab

Figure 1. The AWCD of soil microbial communities in rhizosphere and non-rhizosphere of different plants.

Note: PA-R, rhizosphere soil of Pennisetum alopecuroides; PA-S, non-rhizosphere soil of Pennisetum alopecuroides; AH-R, rhizosphere soil of Arthraxon hispidus; AH-S, rhizosphere soil of Arthraxon hispidus; PL-R, rhizosphere soil of Pueraria lobata; PL-S, rhizosphere soil of Pueraria lobata.

Table 3. Diversity indices for soil microbial communities.

Sample code	Shannon–Wiener Index(H)	Evenness Index(E)	Richness Index(S)	Simpson’s diversity Index (D)	McIntosh Index(U)
PL-R	3.20 ± 0.04Aa	0.93 ± 0.01Aa	23.00 ± 1.53Bb	0.97 ± 0.00Aa	5.00 ± 0.14Aa
PL-S	2.82 ± 0.07Bb	0.79 ± 0.04Bb	27.33 ± 1.76Aa	0.94 ± 0.01Ba	3.01 ± 0.19Bc
AH-R	3.24 ± 0.03Aa	0.94 ± 0.01Aa	30.00 ± 1.00Aa	0.96 ± 0.00Aa	5.53 ± 0.10Aa
AH-S	3.06 ± 0.03Ba	0.88 ± 0.02Ba	24.67 ± 3.18Bab	0.95 ± 0.00Ba	4.82 ± 0.00Ba
PA-R	2.59 ± 0.10Bb	0.73 ± 0.01Bb	28.33 ± 2.52Aa	0.92 ± 0.01Ab	3.63 ± 0.39Ab
PA-S	2.85 ± 0.09Ab	0.90 ± 0.02Aa	22.00 ± 1.20Bb	0.93 ± 0.09Aa	3.58 ± 0.59Ab

Table 4. Utilization of different carbon sources by microbial communities.

Sample code	Carbohydrate	Carboxylic acid	Amino acid	Phenolic compounds	Polymer	Amine
PL-R	0.60 ± 0.02Ab	0.30 ± 0.04Ac	0.72 ± 0.05Ab	0.24 ± 0.03Ab	0.52 ± 0.01Aab	0.54 ± 0.04Aa
PL-S	0.37 ± 0.01Bb	0.05 ± 0.00Bc	0.48 ± 0.03Bb	0.05 ± 0.00Bb	0.04 ± 0.01Bc	0.13 ± 0.01Bb
AH-R	0.79 ± 0.04Aa	0.52 ± 0.07Aa	0.92 ± 0.05Aa	0.49 ± 0.04Aa	0.57 ± 0.04Ba	0.54 ± 0.11Aa
AH-S	0.50 ± 0.02Ba	0.28 ± 0.01Bb	0.66 ± 0.06Ba	0.80 ± 0.03Ba	0.64 ± 0.01Aa	0.32 ± 0.00Ba
PA-R	0.42 ± 0.05Ac	0.13 ± 0.02Bc	0.19 ± 0.01Bc	0.07 ± 0.01Ac	0.42 ± 0.09Aa	0.00 ± 0.00Bb
PA-S	0.27 ± 0.02Bc	0.46 ± 0.03Aa	0.31 ± 0.06Ac	0.00 ± 0.00Bc	0.48 ± 0.06Ab	0.01 ± 0.00Ac

Figure 2. PCA analysis of carbon source utilization in soil microbial community.

Note: PA-R, rhizosphere soil of Pennisetum alopecuroides; PA-S, non-rhizosphere soil of Pennisetum alopecuroides; AH-R, rhizosphere soil of Arthraxon hispidus; AH-S, rhizosphere soil of Arthraxon hispidus; PL-R, rhizosphere soil of Pueraria lobata; PL-S, rhizosphere soil of Pueraria lobata.

Table 5. Major carbon sources significantly associated with PC1 in soil.

Carbon sources	Category	R
L-Asparagine	Amino acid	0.968
2-Hydroxy benzoic acid	Carboxylic acid	0.909
L-Phenylalanine	Amino acid	0.949
4-Hydroxy benzoic acid	Carboxylic acid	0.927
L-Serine	Amino acid	0.966
Phenylethyl-amine	Amine	0.898
a-D-Lactose	Carbohydrate	0.931
D-Malic acid	Carboxylic acid	0.898
Putrescine	Amine	0.936

Table 6. Soil related factor matrix.

	Shannon–Wiener Index(H)	Evenness Index(E)	Richness Index(S)	Simpson’s diversity Index(D)	McIntosh Index(U)
Organic matter (OM)	0.624*	0.469	−0.048	0.740**	0.715**
Total nitrogen (TN)	−0.582	−0.625*	0.271	−0.423	−0.462
Available nitrogen (AN)	0.416	0.316	−0.353	0.625*	0.318
Total phosphorus (TP)	0.019	0.020	0.005	0.147	0.167
Available phosphorus (AP)	−0.245	−0.224	0.671*	−0.456	−0.053
Total Potassium (TK)	0.577	0.281	0.380	0.591	0.380
Available Potassium (AK)	0.185	−0.065	0.619*	0.130	0.113
Potential of hydrogen (pH)	−0.625*	−0.383	0.135	0.852**	−0.452
Moisture content (WC)	−0.130	−0.283	0.167	0.123	−0.268
Microbial biomass carbon (MBC)	0.421	0.458	−0.296	0.452	0.621*
Microbial biomass nitrogen (MBN)	0.417	0.286	0.083	0.549	0.541
Microbial biomass phosphorus (MBP)	0.352	0.329	0.567	0.144	0.649*

Figure 3. RDA analysis of carbon source metabolism and soil properties in soil microbial community.

Note: OM, organic matter; TN, total nitrogen; AN, available nitrogen; TP, total phosphorus; AP, available phosphorus; TK, total potassium; AK, available potassium; pH, potential of hydrogen; WC, moisture content; MBC, microbial biomass carbon; MBN, microbial biomass nitrogen; MBP, microbial biomass phosphorus; PA-R, rhizosphere soil of Pennisetum alopecuroides; PA-S, non-rhizosphere soil of Pennisetum alopecuroides; AH-R, rhizosphere soil of Arthraxon hispidus; AH-S, rhizosphere soil of Arthraxon hispidus; PL-R, rhizosphere soil of Pueraria lobata; PL-S, rhizosphere soil of Pueraria lobata.

Data availability statement

The data that support the findings of this study are openly available in [repository name “Data of soil microbial functional diversity between rhizosphere and non-rhizosphere of different dominant species in vegetation concrete slope”] at https://www.scidb.cn/s/RZviAb, reference number [10.11922/sciencedb.01393].