Gene silencing of VP1 gene of coxsackievirus B3 neurotropic strain Nancy by dsRNAs and siRNAs

Figures & data

Table 1. Primers used in touch-down RT-PCR and synthesis of dsRNA.

#	Primer	Sequence (5’→ 3’)	Length (nt)	Position in the genetic region	Length of fragment (bp)
1	VP1b F	C GAC ATC TTG TGC TTC GTA TCG	22	2361-2382	1000
2	VP1b R	CGC GGG TGG CGA GGT GTC TAT	21	3361-3341
3	VP1a F	GGC CCA GTG GAA GAA TCG GT	20	2452-2471	800
4	VP1a R	GTG GTG ACT CCT GTT GGG TT	20	3260-3241
5	VP1b F DS	TAA TAC GAC TCA CTA TAG GGC GAC ATC TTG TGC TTC GTA TCG	42	2361-2382	1080
6	VP1b R DS	GGA AAA AAA CGC GGG TGG CGA GGT GTC TAT	30	3361-3341
7	VP1a F DS	TAA TAC GAC TCA CTA TAG GGG GCC CAG TGG AAG AAT CGG T	40	2452-2471	820
8	VP1a R DS	GGA AAA AAA ACA CTG GTA GCG GTA CTG G	28	3260-3242

Figure 1. Cytotoxicity assay of different concentrations of dsRNAs (A) and siRNAs (B) specific for two sequences (a and b) of the VP1 genetic region of CVB3 in HEp-2 cells transfected with Oligofectamine™.

Note: Values are mean from four replicates.

Figure 2. Antiviral assay of different concentrations of dsRNAs (A) and siRNAs (B) specific for two sequences (a and b) of the VP1 genetic region of CVB3 in HEp-2 cells transfected with Oligofectamine™.

Note: Values are mean from four replicates, rounded to an integer, with standard deviation error bars.

Data availability statement

The authors confirm that the data supporting the findings of this study is available within the article.