Effect of exogenous melatonin on salt stress in cucumber: alleviating effect and molecular basis

Figures & data

Table 1. List of primers used in the present study.

Gene name	Forward primer (5’-3’)	Reverse primer (5’-3’)
Actin	TTCTGGTGATGGTGTGAGTC	GGCAGTGGTGGTGAACATG
Csa1G150	TCGAATGGGCCTTGAAGCTC	CGACTCGGAGATGGCTTTGT
Csa3G160	GTCATCGGAGCGATTGGGAT	GACGAATACAAAGACGCCGC
Csa1G440	AATCATGCCCCGACGTATCC	AAGCTCACTCACCACACCAG
Csa2G050	ATTTCCGCCGAGTCAACCAT	CCATCGACGGCGGAGTTATT
Csa2G530	GGAGAGCATTTGGCTCCTGA	TCAGTAGGCAGCAAAGGACG
Csa4G610	AATCATGCCCCGACGTATCC	AAGCTCACTCACCACACCAG

Figure 1. Effects of different concentrations of melatonin on ASA (A), proline (B), GST (C), GSH (D), SOD (E), POD (F), polyphenol (G), flavonoid (H), souble protein (I) and soluble sugars (J) in leaves of salt stressed cucumbers. Data are presented as means ± SD. Different letters (a, b, c, d) above the error bars indicate significant differences among the treatments (p < 0.05).

Figure 2. Volcano plot of DEGs in melatonin-treated groups and the control.

Figure 3. KEGG pathway enrichment analysis for DEGs in the control and melatonin-treated groups.

Figure 4. Relative expression level of Cas1G150 (A), Cas3G160 (B), Cas1G440 (C), Cas2G050 (D), Cas2G530 (E) and Cas4G610 (F). Data of quantitative real-time PCR are presented as means ± SD.

Data availability statement

The authors confirm that the data supporting the findings of this study are available upon reasonable request. Data and calculation tools are available from the corresponding author (SZ).