siRNAs based gene silencing of potato virus Y by simultaneous blocking of HC-Pro and NIa

Figures & data

Figure 1. The organisation of PVY viral genome. The boxes represent the genetic regions of PVY encoding the virus proteins: P1, protein 1; HC-Pro, helper component proteinase; P3, the third protein; CI, protein of cylindrical inclusion; 6K1 and 6K2, 6 K proteins; VPg, viral genome-linked protein; NIa, nuclear inclusion protein a; NIb, nuclear inclusion protein b (RNA-dependent-RNA polymerase); CP, coat protein (based on the findings of Riechmann et al. 1992; Revers et al. 1999).

Figure 2. DAS-ELISA of potato plants after treatment with combinations of dsRNAs and siRNAs, specific for HC-Pro and Nia of PVY^N/NTN and after infection. K- H₂O, healthy control plants treated with pure water; dsRNAs HC-Pro-NIA, potato plants treated with a mixture of dsRNAs specific for the HC-Pro-region and dsRNAs specific for the Nia-region of PVY, and infected; siRNAs HC-Pro-NIA, potato plants treated with a mixture of siRNAs specific for the HC-Pro-region and siRNAs specific for the Nia-region of PVY, and infected; K- healthy potato plants, not treated and not infected; K + infected with PVY potato plants, not treated; K + kit, positive control of the ELISA kit; K- kit, negative control of the ELISA kit; K- buffer, negative buffer control of the ELISA. 1, 2 and 3 refer to the number of the pot. Note: Values are means from four replicates of each pot; error bars show standard deviation (±SD).

Riechmann J, Lain S, Garcia JA. Highlights and prospects of potyvirus molecular biology. J. Gen. Virol. 1992;73(1):1–16.

 PubMedGoogle Scholar

Revers F, Le Gall O, Candresse T, et al. New advances in understanding the molecular biology of plant/potyvirus interactions. MPMI. 1999;12(5):367–376.

 Web of Science ®Google Scholar

Data availability statement

The data supporting the findings in this study are available from the corresponding author upon reasonable request.