Association between angiotensinogen M235T gene polymorphism and risk of ischemic stroke among the Ethiopian population: a case control study

Figures & data

Figure 1. Agarose gel (1%) electrophoresis showing the quality of isolated genomic DNA. Lane 1: 100 bp DNA ladder (Ready to Load, Solis BioDyne, Tartu, Estonia); Lanes 2 – 13: isolated genomic DNA.

Figure 2. Representative 2% agarose gel electrophoresis showing PCR products of the AGT M235T gene. Lane 1: homozygous MM genotype; Lanes 2 and 3: heterozygous MT genotypes; Lanes 4 and 5: homozygous TT genotypes; Lane 6: 100 bp DNA ladder.

Table 1. General characteristics of the study participants in Debre Tabor Referral Hospital, Northwest Ethiopia, 2022.

Variables	IS (n = 36)	Control (n = 36)	P-value
Sex (M/F)	19/17	18/18	0.8136
Age (yr)	59.4 ± 12.1	58.2 ± 6.9	0.6177
BMI (kg/m^2)	23.6 ± 4.7	23.1 ± 4.2	0.6471
SBP (mmHg)	147.4 ± 6.0	116.2 ± 3.9	< 0.001*
DBP (mmHg)	91.3 ± 3.8	76.0 ± 4.0	< 0.001*
FBG (mg/dL)	91.0 ± 18.9	89.9 ± 5.7	0.7503
Total Cholesterol (mg/dL)	190.5 ± 47.6	153.4 ± 46.2	< 0.001*
Triglyceride (mg/dL)	135.4 ± 37.3	105.5 ± 37.1	< 0.001*
LDL-Cholesterol (mg/dL)	94.7 ± 32.6	68.5 ± 28.2	< 0.001*
HDL-Cholesterol (mg/dL)	43.8 ± 8.2	55.5 ± 11.5	< 0.001*
Creatinine (mg/dL)	0.79 ± 0.13	0.75 ± 0.12	0.2214
Family history of IS (%)	14.29 %	21.14 %	0.1423

Note: ^*Statistically significant differences at p < 0.05.

Figure 3. Distribution of the AGT M235T genotype in cases and controls.

Note: * Statistically significant differences at P < 0.05.

Table 2. Distribution of AGT M235T genotypes and allele frequencies of the study participants in Debre Tabor Referral Hospital, Northwest Ethiopia, 2022.

Genotype	IS (n = 36)	Control (n = 36)	OR (95% CL)	p-value
TT	19 (52.78 %)	9 (25.00 %)	4.64 (1.23–17.41)	0.023*
MT	12 (33.33 %)	16 (44.44 %)	1.65 (0.45–6.02)	0.449
MM	5 (13.89 %)	11 (30.56 %)	Ref
Allele frequency
T	50 (69.44 %)	34 (47.22 %)	2.54 (1.28–5.02)	0.003*
M	22 (30.56 %)	38 (52.78 %)	Ref

Note: ^*Statistically significant differences at p < 0.05.

Table 3. Association of AGT M235T genotype with clinical characteristics in Debre Tabor Referral Hospital, Northwest Ethiopia, 2022.

Variables	Genotypes
	TT (n = 28)	MT (n = 28)	MM (n = 16)	p-value
Sex (M/F)	16/18	14/12	7/5	0.6479
Age (yr)	60.4 ± 10.4	58.1 ± 9.5	57.3 ± 9.6	0.3792
BMI (kg/m^2)	23.2 ± 4.9	23.5 ± 4.3	23.2 ± 4.1	0.4352
SBP (mmHg)	138.3 ± 14.9	129.0 ± 17.1	125.5 ± 14.9	0.2315
DBP (mmHg)	87.4 ± 7.7	81.9 ± 8.7	80.2 ± 8.0	0.1377
FBG (mg/dL)	92.9 ± 15.6	88.0 ± 9.3	90.4 ± 17.3	0.1956
TC (mg/dL)	177.1 ± 57.1	164.3 ± 38.0	176.2 ± 57.6	0.6323
TG (mg/dL)	135.9 ± 46.9	110.2 ± 35.1	111.6 ± 27.1	0.5058
LDL-C (mg/dL)	90.7 ± 31.5	82.8 ± 37.1	63.7 ± 20.1	0.7218
HDL-C (mg/dL)	46.0 ± 11.4	50.9 ± 10.1	54.0 ± 12.7	0.3389
Creatinine (mg/dL)	0.77 ± 0.10	0.79 ± 0.17	0.75 ± 0.11	0.8423

Note: ^*Statistically significant differences at p < 0.05.

Availability of data

The anonymized data used and/or analyzed in this are available from the corresponding author upon reasonable request.