Expression of membrane-bound β-Klotho on peripheral blood CD4+ and CD8+ T lymphocytes of healthy subjects

Figures & data

Table 1. Absolute counts and proportions of lymphocytes and lymphocytes subset in the studied groups (mean ± SD).

Lymphocyte population	Group A (20–30 years) (n = 12)	Group B (30–50 years) (n = 62)	Group D (>70 years) (n = 18)	Kruskal-Wallis post-hoc Dunn test
Lymphocyte (AC)	2256 ± 436	2213 ± 665	2185 ± 921	p > 0.05
Т-cells (АС)	1532 ± 366	1609 ± 503	1388 ± 510	p > 0.05
Т-cells (% of lymphocytes)	67.87 ± 6.93	72.82 ± 7.11	65.77 ± 13.42	p > 0.05
CD3 + CD4+ T-cells (AC)	924 ± 228	1027 ± 364	1004 ± 507	p > 0.05
CD3 + CD4 T (% of lymphocytes)	41.04 ± 5.88	46.43 ± 7.78	44.82 ± 8.25	p > 0.05
CD3 + CD8 T-cells (AC)	480 ± 156	494 ± 202	528 ± 591	p > 0.05
CD3 + CD8 T-cells (% of lymphocytes)	21.20 ± 5.04	22.25 ± 5.88	21.70 ± 11.13	p > 0.05
CD4/CD8 ratio	2.03 ± 0.53	2.28 ± 0.81	2.45 ± 1.36	p > 0.05
% β-Klotho CD3+ cells	2.21 ± 1.51	0.75 ± 0.68	0.47 ± 0.64	p < 0.0001 A vs. B p = 0.003 A vs. C p = 0.0001 B vs. C p = 0.135
% β-Klotho CD4+ T-cells	3.32 ± 2.61	0.93 ± 1.70	0.72 ± 0.85	p < 0.001 A vs. B p = 0.001 A vs. C p = 0.003 B vs. C p = 1.0
% β-Klotho CD8+ T-cells	6.62 ± 3.92	3.62 ± 2.67	1.57 ± 2.03	p < 0.0001 A vs. B p = 0.048 A vs. C p = 0.0001 B vs. C p = 0.016

Figure 1. Expression of β-Klotho on total CD3+ (A, circles), CD4+ (B, triangles) and CD8+ (C, diamonds) T-cells in healthy subjects. Group A: young adults (open symbols); group B: middle-aged adults (light grey symbols) and group C: elderly (dark grey symbols); ***p < 0.001; **p < 0.01; *p < 0.05; ns, not significant, p > 0.05. Means ± SD are denoted. Comparisons between groups were done by using non-parametric Kruskal–Wallis ANOVA rank tests, followed by post-hoc group pairwise comparisons, with p-value adjustment for multiple comparisons using Dunn’s test.

Figure 2. Inverse correlation between the share of β-Klotho + CD8+ T-lymphocytes and CD27-CD57+ CD8 + T-cells in group B (middle-aged adults) (rho = −0.4, p < 0.01). Correlations were evaluated with Spearman’s rank correlation test.

Data availability statement

The data that support the findings of this study are available from the corresponding author (YT), upon reasonable request.