High cross-pollination rate of Greek oregano (O. vulgare ssp. hirtum) with Common oregano (O. vulgare ssp. vulgare) under open field conditions as revealed by microsatellite marker analysis

Figures & data

Table 1. SSR primer pairs used in the present study. Bold letters indicate tails added to the forward primer (tail M13 – 5′-GTAAAACGACGGCCAGT-3′ and tail C – 5′-CAGGACCAGGCTACCGTG-3′), ta - annealing temperature.

SSR locus	Repeat motif	Primer	Ta (°C)
R-6M	(TTG)16	FW – 5′-GTAAAACGACGGCCAGTCTGTCGATGCCACTTCTTCAC-3′	54
		Rev – 5′-TCAGGTGAAGCTACTACCCAC-3′
R-38C	(CT)17	FW – 5′-CAGGACCAGGCTACCGTGAATATTTTCAGCCGACTCTTCG-3′	57
		Rev – 5′-CCGTCACGCTTACCTTTTGG-3′
R-40C	(ATCT)9	FW – 5′-CAGGACCAGGCTACCGTGAACTTTAGACACGGATGCGG-3′	57
		Rev – 5′-TGCATTTGCACGTAACTTTCTAC-3′
R-103C	(GA)13	FW – 5′-CAGGACCAGGCTACCGTGAAAAGGCGGCTGCTGATTAC-3′	57
		Rev – 5′-CCCAAGTTCTTGCGAACAGG-3′
R-105C	(GA)17	FW – 5′-CAGGACCAGGCTACCGTGTTGGAGGCTTACTGTCTGGG-3′	57
		Rev – 5′-ATGTTGGGAGCTTTCATGGC-3′
R-115C	(AG)15	FW – 5′-CAGGACCAGGCTACCGTGCCATGGCTTCCGATTTGAGC-3′	57
		Rev – 5′-GCAAATTAATCAAACGGTAAACTGTC-3′

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.