Identification of QTL controlling the ratio of linalool to linalyl acetate in the flowers of Lavandula angustifolia Mill var. Hemus

Figures & data

Figure 1. Mapping of L/LA-QTL and allele configuration of loci in the L/LA-QTL region in the recombinant plants. (a) LOD profile and molecular marker locations in the L/LA-QTL region on LG8 (the results of QTL analysis for all LGs of the L. angustifolia genome are presented in Supporting Information S1); (b) genotypes of plants according to the alleles of SSR, SRAP and ATT4 loci located in the L/LA-QTL region. The plant names correspond to the names of groups of plants from the segregating population which are presented in Table 1. The low/high value of the L/LA ratio is indicated for each plant genotype.

Figure 2. Box and whiskers plot showing the distribution of data for the L/LA ratio among groups of plants with different zygosity state for the L/LA-QTL region.

Table 1. Allele configurations of loci located in the identified L/LA-QTL region, L/LA ratio, relative contents of linalool (L) and linalyl acetate (LA) and total relative content of the sum of linalool and linalyl acetate (L + LA). The names of groups of plants with the same genotypes for the analyzed loci in the L/LA-QTL region are shown in bold.

Loci (Alleles)	Groups of plants with specific allele combinations of loci within the L/LA-QTL region
	AA	AB	BB	R1	R2	R3	R4	R5	R6
123 (A = 252, B = 244)	AA	AB	BB	AA	AA	AB	AB	AB	AA
257* (A = 0, B = 115)	AA	AB/BB*	AB/BB	AA	AA	AB/BB	AB/BB	AA	AB/BB
LiAAT4 (A = T&A, B = C&G)	AA	AB	BB	AA	AA	AA	AB	AB	AB
E6M4-177* (A = 0, B = 177)	AA	AB/BB	AB/BB	AA	AB/BB	AB/BB	AB/BB	AB/BB	AA
481A (A = 257, B = 247)	AA	AB	BB	AB	AB	AA	AA	AA	AB
Number of plants	41	54	124	2	8	1	1	1	2
L/LA ratio	1.18^a	0.56 ^b	0.39^c	1.04	1.22	0.99	0.40	0.32	0.43
	± 0.35	± 0.22	± 0.11	± 0.08	± 0.23				± 0.06
L (%)	39.18^a	25.71^b	20.80^c	36.96	38.08	40.70	16.07	15.26	19.94
	± 5.77	± 6.33	± 4.81	±4.04	±6.61				±0.48
LA (%)	34.69^a	47.70^b	53.97^c	39.88	31.67	41.00	40.24	48.11	46.93
	± 6.31	± 7.15	± 6.18	±0.87	±5.51				±5.96
L + LA (%)	76.98^a	73.41^a	74.77^a	76.84	69.73	81.70	56.31	63.37	66.87
	± 7.09	± 7.72	± 7.24	±4.91	±9.99				±5.48

Note: Mean values for AA, AB and BB groups for L/LA, L, LA and L + LA which do not share one and the same letter are significantly different (p < .001).

*The SSR marker 257 and SRAP marker E6M4-177 have null allele presented as ‘0’. Accordingly, their alleles are presented as AB/BB in the plant genotypes showing the pointed SSR or SRAP fragments.

Data availability statement

All data included in this study are available from the corresponding author [I.A.] upon request.