Evaluation of the viability of the Bulgarian BCG vaccine by the adenosine triphosphate assay

Figures & data

Figure 1. Chemiluminescent calibration curve in the range 10, 5, 2.5, 1.25, 0.625, 0.3125 pmol ATP (A) and 1.25, 0.625, 0.3125 pmol ATP and the calculation of the results according to the linear equation (B).

Table 1. Concentrations of ATP [pmol] in different ampoules of two BCG vaccine samples.

ATP [pmol]	BCG vaccine Lot 287-2				BCG vaccine Lot 254-2
Dilution	Amp I	Amp II	Amp III	Amp IV	Amp I	Amp II	Amp III	Amp IV
1\1	0.409	0.606	1.338	0.793	0.18	1.104	0.516	0.559
1\2	0.251	0.223	0.704	0.442	0.167	0.926	0.28	0.282
1\4	0.197	0.165	0.414	0.283	0.063	0.576	0.113	0.139

Table 2. Number of culturable particles (NCP) × 10⁶/mL.

Lot No.	NCP × 10^6/mL
Lot 287-2	2.325	2.951	3.184	3.150	3.250	2.917	3.300	2.483	3.233	2.766	3.067
Lot 254-2	3.012	3.007	2.543	3.063	1.438	3.330	2.930	3.264	2.917	2.603	2.317

Data availability statement

The data are available on request from the corresponding author [E.P.] upon reasonable request.