Salinity induced alterations in ionic concentration of haemolymph and its effects on histopathology of gills and digestive gland in razor clam (Solen dactylus von Cosel, 1989; Bivalvia, Solenidae)

ABSTRACT

Solen dactylus is one of the most common razor clams in the tidal zone in the western Indian Ocean and Persian Gulf. Habitats of these clams may be subject to salinity fluctuations due to high evaporation and heavy rainfall. To assess their adaptation, in the laboratory clams were placed in 50-litre tanks with 40 cm depth of natural substrate and were kept in salinity of 45 ppt for a week before subsequent experiments. They were then exposed to salinities of 5, 20, 35, 45 and 65 ppt for three weeks. Concentrations of Na⁺, Cl^–, K⁺, Ca²⁺, and Mg²⁺, as well as osmolality in the haemolymph and tank water were measured at 1, 24, 72 h, one week and three weeks after the start of the experiment. Haemolymph Na⁺ and Cl^– values followed the concentrations of the external medium so that they decreased at low salinities and increased at high salinities. The Ca²⁺, Mg²⁺ and K⁺ ions all showed a common pattern, and their trend was independent of the external environment. Histopathological analysis showed severe tissue damage at low salinities with expanded intercellular spaces, an increase of intracytoplasmic vacuoles in the digestive tubules, and necrosis and destruction of lamellae in gill tissues.

KEYWORDS:

• Osmoconformer
• marine invertebrates
• salinity stress
• functional responses
• molluscan physiology

Acknowledgements

The authors thank the Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, for laboratory facilities and scientific advice. We also thank the respectful referees, editors and reviewers for their valuable comments on previous versions of manuscript. This work was funded and supported by Khorramshahr University of Marine Sciences and Technology (grant number 9434404). All individual rights, animal rights and scientific ethics are observed in this study.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was funded and supported by Khorramshahr University of Marine Sciences and Technology [grant number 9434404].