Figures & data
Figure 1. Frequency distributions of (a) max root diameter (MRD) in the pot experiment and (b) average root diameter (ARD) in the field experiment in the BC1F1 population. (a) The DNA of 20 individuals with low MRD (5.5 mm < MRD < 7.5 mm, LP) and the DNA of 20 individuals with high MRD (MRD > 12.5 mm, HP) were bulked as LP-bulk and HP-bulk in the pot experiment, respectively. (b) The DNA of 20 individuals with low ARD (7.0 mm < ARD < 12.0 mm, LF) and the DNA of 20 individuals with high ARD (ARD > 18.0 mm, HF) were bulked as LF-bulk and HF-bulk in the field experiment, respectively.
Table 1. Sequences of primers in DNA markers designed for qRT1.
Figure 2. Tricube-smoothed Δ(SNP-index) with statistical confidence intervals (light blue, 99%; red, 95%) (a) between LP-bulk and HP-bulk in the pot experiment and (b) between LF-bulk and HF-bulk in the field experiment. The major QTL (qRT1) identified on chr06 of Itr_r2.2 in both the pot and field experiments by QTL-seq is highlighted in yellow.
Figure 3. Max root diameter (MRD) in BC2F2 population and Mx23Hm. A, H, and B indicate BC2F2 individuals with Mx23Hm-type homozygous alleles, heterozygous alleles, and 0431-1-type homozygous alleles in the three markers (InDel_4.95, InDel_5.73, and InDel_6.86), respectively. R indicates BC2F2 individuals with recombinant alleles among the three markers. Values followed by the same letter are not significantly different according to Tukey’s honestly significant difference (HSD) test (P < 0.05).
