https://orcid.org/0000-0001-7403-6493
Figures & data
Figure 1. Effect of cigarette smoke (CS) on levels of ROS and autophagy markers in the lungs of mice exposed to CS for different durations (n = 10 per group). Dichlorofluorescein (DCF) level (a), SESN2 protein levels (b), AMPK and mTOR phosphorylation (c), ULK1 phosphorylation (d), ATG12 protein levels (e), LC3B protein levels (f), and representative western blot analysis of the evaluated proteins (g) in control (non-exposed group) and for different days of CS exposure (7, 15, 30, 45, and 60 days). Data are expressed as the mean and standard error of the means (mean ± SEM) and analyzed statistically by one-way analysis of variance, followed by Tukey’s HSD post hoc test (*p < 0.05 versus control).
Figure 2. Effect of antioxidant NAC supplementation on ROS and autophagy markers in the lungs of mice exposed to cigarette smoke (CS) for 45 days (n = 10 per group). Dichlorofluorescein (DCF) level (a), protein levels of SESN2 (b), AMPK phosphorylation (c), mTOR phosphorylation (d), ULK1 phosphorylation (e), ATG12 (f), LC3B (g), and representative western blot of the considered proteins (h) in the control (non-exposed group), control plus NAC, CS exposure for 45 days without NAC, and 45 days of CS exposure with NAC. Data are expressed as the mean and standard error of the means (mean ± SEM) and analyzed statistically by one-way analysis of variance, followed by Tukey’s HSD post hoc test. (*p < 0.05 versus control and control + NAC; #p < 0.05 versus 45 + NAC).
Figure 3. Effect of cigarette smoke (CS) cessation on ROS and autophagy markers in the lungs of mice after 45 days of CS exposure (n = 10 per group). Dichlorofluorescein (DCF) level (a), protein levels of SESN2 (b), AMPK phosphorylation (c), mTOR phosphorylation (d), ULK1 phosphorylation (e), ATG12 (f), LC3B (g), and representative western blot analysis of the evaluated proteins (h) in the control (non-exposed group), CS exposure for 45 days, and 45 days CS exposure followed by CS cessation for different days (7, 15, 30, and 45 days) groups. Data are expressed as the mean and standard error of the means (mean ± SEM) and analyzed statistically by one-way analysis of variance, followed by Tukey’s HSD post hoc test (*p < 0.05 versus cont; #p < 0.05 versus 45 CS).
