Gene expression of insulin receptor, insulin-like growth factor increases and insulin-like growth factor-binding protein-3 reduces with increase in prostate size in benign prostatic hyperplasia

Figures & data

Table 1. Primers used for real-time PCR.

Gene		Primer sequence
IR-A	Forward	GTTTTCGTCCCCAGGCCATC
	Reverse	CCAACATCGCCAAGGGACCT
IR-B	Forward	CACTGGTGCCGAGGACCCTA
	Reverse	GACCTGCGTTTCCGAGATGG
IRS-1	Forward	TATGCCAGCATCAGTTTCCA
	Reverse	TTTGCTGAGGTCATTTAGGTCTT
IRS-2	Forward	CAGGTGTCGGGAGTCGAG
	Reverse	GGAGGAGAAGGTCTCGGAAC
IGF-1	Forward	TGTGGAGACACGGGCTTTTA
	Reverse	ATCCACGATGCCTGTCTGA
IGF-2	Forward	CCCCCGAAAAGTACAACATC
	Reverse	GGGGGAGAATTCGTCTGATT
IGFBP-3	Forward	AAGTTGACTACGAGTCTCAG
	Reverse	AATCAGTTCACCACAAACAGA
IGF-1R	Forward	GAGCAGCTAGAAGGGAATTAC
	Reverse	AAGTTCTGGTTGTCGAGGA
GAPDH	Forward	AGCCACATCGCTCAGACAC
	Reverse	GCCCAATACGACCAAATCC
ACTIN	Forward	ATCTGGCACCACACTTCTACA
	Reverse	GTTTCGTGGATGCCACAGGACT

Table 2. Mean and S.D or Median (range) of age, prostate size and biochemical parameters among BPH groups.

Parameters	BPH patients with prostate size more than 30 ml (Group A) (n = 13)	BPH patients with prostate size less than 30 ml (Group B) (n = 14)	p value
Age (years)	60 ± 9.6	62 ± 10.2	.6
BMI	22.3 ± 2.2	20.1 ± 3.8	.44
Glucose (mg/dL)	101 ± 10.9	99.7 ± 11.9	.81
Insulin (µIU/mL)	18.9 ± 7.3	26.8 ± 8.2	.04
Insulin-like growth factor 1 (µg/L)	123.3 ± 7.3	123.6 ± 7.8	.91
Insulin-like growth factor binding protein 3 (µg/L)	1093.2 ± 215.7	862.8 ± 169.4	.02
Prostate-specific Antigen (µg/L)	0.8 (0.3–3.7)	4.2 (0.6–12.7)	.01

p value <.05 is considered as significant.

Figure 1. (A) Relative fold change of IR A and IR B in prostate tissues of BPH patients (Group A: Prostate size <30 Ml, Group B: Prostate size >30 ml). Data were expressed in Mean ± SEM. *p = .007 for IR A, p = .01 for IR B. (B) Relative fold change of IRS-1 and IRS-2 in prostate tissues of BPH patients (Group A: Prostate size <30 Ml, Group B: Prostate size >30 ml). Data were expressed in Mean ± SEM. p value – .12 for IRS-1, p value – .25 for IRS-2.

Figure 2. Relative fold change of IGF-1, IGF-2, IGF-1 R and IGFBP-3 in prostate tissues of BPH patients (Group A: Prostate size <30 Ml, Group B: Prostate size >30 ml). Data were expressed in Mean ± SEM. *p = .001 for IGF-1, p = .003 for IGF-2, p = .003 for IGFBP-3.

Figure 3. (a) Relative ratio of IR-B/Beta actin protein expression in prostate tissues of BPH patients. Data were expressed in Mean ± SEM, *indicates statistically significant, p values – .05 for IR-B. (b) First line (C1) represent Group A (<30 ml prostate size), next four lines (S1, S2, S3, S4) represents Group B (>30 ml prostate size).