MicroRNA-copy number variations in coronary artery disease patients with or without type 2 diabetes mellitus

Abstract

Background

An important cause of Coronary Artery Disease (CAD) is Type 2 Diabetes Mellitus (T2DM). The aim of this study was the evaluation of copy number variations (CNVs) of hsa-miR-93, hsa-miR-122, hsa-miR-192 in CAD patients with or without T2DM.

Methods

CNVs of three micro-RNAs in 50 CAD patients and 50 non-CAD subjects both with and without diabetes were evaluated by real-time PCR and compared in three comparison groups namely 1, 2 and 3 (including comparison between CAD and non-CAD, diabetic CAD and non-diabetic CAD and between diabetic CAD and diabetic non-CAD subjects, respectively).

Results

There were significant differences in CNVs of hsa-miR-93 between cases and controls in comparison groups 1 and 3 (p = .0310 and .0232, respectively), for hsa-miR-122 in all comparison groups, and for hsa-miR-192 in comparison group 3 (p = .0181).

Conclusion

We showed the association of these microRNA-CNVs with CAD, T2DM or both simultaneously.

Keywords:

• Coronary artery disease
• type 2 diabetes mellitus
• copy number variation
• microRNA
• quantitative real-time PCR

Acknowledgements

This article has been extracted from the thesis written by Mrs nasim sohrabifar in School of Medicine Shahid Beheshti University of Medical Sciences. (Registration No: M45).

Human subjects/informed consent statement

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study.

Disclosure statement

The authors declare that no conflict of interest occurs for this work.