3-Bromopyruvate elevates ROS and induces hormesis to exert a caloric restriction mimetic effect in young and old rats

Abstract

Context

3-Bromopyruvate (3-BP) is a glycolytic inhibitor and a putative caloric restriction mimetic.

Objective

We have examined the effect of low-dose administration of 3-BP to rats and assess the CRM effect by measuring an array of biomarkers of oxidative stress.

Materials and methods

Male Wistar young and old rats were administered with a low-dose 3-BP for four weeks.

Results

A significant increase in ROS was observed in 3-BP-treated rats (both young and old), an increase in erythrocyte PMRS (plasma membrane redox system), FRAP (Ferric reducing ability of plasma), catalase and superoxide dismutase activities were also observed. Treatment with 3-BP also reduced protein carbonyl, advanced oxidation protein products, plasma sialic acid, and advanced glycation end products.

Conclusion

Short-term 3-BP treatment can provide protection against oxidant stress. We suggest that 3-BP triggers a hormetic response subsequent to an increase in ROS leading to the induction of a protective defense mechanism.

Keywords:

• Aging; CRM; oxidative stress; erythrocyte; hormesis

Disclosure statement

The authors declare no conflict of interest.

Data availability statement

The data that support the findings of this study are available from the corresponding author, [SIR], upon reasonable request.

Additional information

Funding

This work was supported by a research grant to SIR from the SERB, Department of Science and Technology, Govt of India [EMR/2016/006470]. We would also like to acknowledge Dr. D.S. Kothari Post-Doctoral Fellowship scheme of University Grant Commission (UGC), New Delhi, India, for providing financial support [F(0).4–2/2006 (BSR)/BL/17–18/0381] to Shambhu Sharan Tripathi.