EDC and sulfo-NHS functionalized on PVC-g-PEGMA for streptokinase immobilization

Figures & data

Figure 1. Grafting of PEGMA onto PVC films with increasing exposure time in hours, reaction time of 23 h and dose rate of 17.3E + 17 quantum cm⁻² s⁻¹. (a) Different temperatures and 0.2 mol/L of monomer concentration. (b) Different monomer concentrations and reaction temperature of 70 °C.

Figure 2. AFM at different graft of a partially graft was very important to have an overview of the process.

Figure 3. ATR-FTIR spectra of the starting and modified: PVC film, PEGMA, and copolymer.

Table 1. Contact angle of PVC-g-PEGMA vs. graft value. All measures were taken in water at 26 °C and pH 7.

Graft	Contact angle
0	86 ± 3
0.15	72 ± 3
0.25	68 ± 3
0.37	71 ± 3
0.48	68 ± 3
0.82	70 ± 3

Figure 4. Fluorescence microscope picture of (a) graft copolymer alone and (b) SK previously coupled with FITC and immobilized onto graft copolymer.

Figure 5. Effect of graft yield on immobilization of SK.

Figure 6. AFM of PVC, graft copolymer, and SK immobilized onto graft copolymner.

Figure 7. AFM average values of radius and height: (a) r = 0.6 μm, H = 250 nm, (b) r = 1.1 μm, H = 360 nm, and (c) r = 2.9 μm, H = 500 nm.