Comparative Nematocidal Activity of Chromatographic Fractions of Leucaena leucocephala. Seed Against Gastrointestinal Sheep Nematodes

Figures & data

Figure 1 Concentration-response curve of chromatographic fractions of L. leucocephala. seed against infective larvae of H. contortus. using global model of nonlinear regression curve-fitting.

Figure 2 Relative efficacy of chromatographic fractions of L. leucocephala. seed.

Table 1.. LC₅₀ of L. leucocephala. test chromatographic fractions using global model of nonlinear regression curve-fitting.

	LC50^a.(µg/ml)
Fractions	Best-fit value	95% CI	Std. error	R^2
A	77.66	72.62 to 83.04	1.03	0.951
B	65.56	62.17 to 69.13	1.03	0.968
C	56.72	53.73 to 59.89	1.03	0.973
D	40.80	37.26 to 44.68	1.05	0.953

^a.Global shared parameters:

Constraints.: Top is shared and < 1.0, hill slope is shared, bottom is shared and > 0.0.

Best fit value.±SE (SE = standard error).: Bottom = 0.110±0.057; top = 0.921±0.017; hill slope = − 2.460±0.279.

Goodness of fit.: Degrees of freedom = 65; absolute sum of squares = 0.120; Sy.x = 0.043; R² = 0.967.

Table 2.. Tukey's multiple comparison test comparing the LC₅₀ values of chromatographic fractions of L. leucocephala seed..

Comparisons	p value
Fraction A vs. fraction B	p < 0.01
Fraction A vs. fraction C	p < 0.001
Fraction A vs. fraction D	p < 0.001
Fraction B vs. fraction C	p < 0.01
Fraction B vs. fraction D	p < 0.001
Fraction C vs. fraction D	p < 0.001

Figure 3 Chemical structures of catechin and mimosine.