GC-MS Analysis of Chloroform Extracts in Flowers, Stems, and Roots of Tripleurospermum callosum.

Figures & data

Table 1.. Lipid components in the flowers, stems, and roots of T. callosum. by GC-MS.

No.	Compounds	RI	Flower (%)	Stem (%)	Root (%)
1.	2-Methyl-naphthalene	1208	0.26	0.23	0.73
2.	(4α-trans.)-Decahydro-4α- methyl-1-methylene-7-(1-methylethylidene)-naphthalene	1281	—	—	0.45
3.	Isocomene	1347	0.14	0.52	3.07
4.	trans.-β-Farnesene	1469	0.45	—	—
5.	1-Pentadecene	1513	—	—	0.69
6.	p.-Methoxy-β-cyclopropylstyrene	1543	0.70	0.28	4.08
7.	(+)-Spathulenol	1578	0.28	0.14	2.42
8.	(−)-Caryophyllene oxide	1581	—	—	0.94
9.	Geranyl isovalerate	1588	—	—	1.04
10.	n.-Hexadecane	1600	0.40	0.07	0.40
11.	Dec-4,6,8-triyn-2-enoic acid, methyl ester	1622	—	—	0.26
12.	4-Methyl-2-(2-methyltetrahydrofuran-2-yl)phenol	1629	—	—	0.54
13.	α-Elemene	1650	—	—	1.15
14.	Cyclododecyne	1662	—	—	0.47
15.	Heptadecane	1700	0.18	0.42	—
16.	6-Isopropenyl-4,8a-dimethyl-1,2,3,5,6,7,8,8a-octahydronaphthalene-2-ol	1714	—	—	0.66
17.	1-(1,1-Dimethylethyl)-4-ethoxy-benzene	1728	—	—	0.75
18.	2-Pentadecanone,6,10,14-trimethyl	1731	—	0.15	—
19.	2-(2-oxoethyl)-cis.-Bicyclo[3.3.0]octane-3,7-dione	1734	—	—	1.35
20.	Tetradecanioc acid	1768	0.33	0.19	0.27
21.	n.-Octadecane	1800	0.13	—	0.24
22.	Isobutylphthalate	1866	0.37	—	—
23.	Pentadecanoic acid	1867	—	—	0.49
24.	2,8-Dimethyldibenzothiophene	1868	0.30	0.43	—
25.	4,9-Dimethyl-naptho[2,3β]thiophene	1896	—	0.21	—
26.	Nonadecane	1900	—	0.07	—
27.	1-Phenyl-3-ethyl-4-hexen-1-yn-3-ol	1906	—	—	2.85
28.	2,5-Dimethyl-phenanthrene	1952	—	0.91	—
29.	Unknown	1992	—	—	7.28
30.	n.-Eicosane	2000	—	0.93	—
31.	n.-Hexadecanoic acid	2024	5.35	17.88	6.18
32.	[1,1′-Diphenyl]-3-amine	2031	—	—	4.71
33.	Tricyclo[7.3.0.0(3,8)]dodec-1(9)-en-12-one,2,2-dicyano, (Z.)	2049	1.35	1.38	—
34.	7-Isopropyl-1,3,4-trimethylazulene	2081	—	—	0.27
35.	Isofraxidin	2085	—	—	1.32
36.	Phytol	2089	—	3.09	—
37.	n.-Heneicosane	2100	0.38	—	0.57
38.	8-epi-1,2-Dihydroartemisin	2107	—	—	4.82
39.	Linoleic acid	2118	3.30	16.18	0.72
40.	Artemisia acetate	2119	—	—	0.44
41.	9,12-Octadecadien-1-ol	2185	0.77	0.62	0.17
42.	Z.,Z.-10,12-Hexadecadienal	2191	—	—	0.29
43.	1,8-Diaza-2,9-diketocyclotetradecane	2196	1.27	—	2.04
44.	8-Oxo-2-nonenal	2197	—	0.64	—
45.	n.-Docosane	2200	0.26	—	0.29
46.	(Z.)-9-Tricosene	2299	0.17	0.49	—
47.	n.-Tricosane	2300	1.46	0.28	—
48.	6-Nitro-cyclohexadecane-1,3-dione	2386	—	0.28	—
49.	(Z.)-9-Octadecenamide	2397	0.28	—	0.78
50.	n.-Pentacosane	2500	4.77	—	0.48
51.	di-(2-Ethylhexyl)phthalate	2519	0.17	—	—
52.	di-n.-Octyl phthalate	2578	—	—	0.43
53.	Cyclotetracosane	2589	0.82	4.05	5.88
54.	n.-Heptacosane	2700	4.52	—	—
55.	1,19-Eicosadiene	2719	0.14	0.27	—
56.	1-Eicosanol	2757	—	4.50	—
57.	1-Nonadecene	2762	0.23	0.28	0.71
58.	11-Butyl-docosane	2779	0.72	—	—
59.	n.-Octacosane	2800	0.32	0.19	—
60.	Squalene	2829	0.64	—	—
61.	n.-Nonacosane	2900	11.04	—	—
62.	(E.)-1-Methoxy-3,7-dimethyl-2,6-octadiene	2910	0.15	—	—
63.	Hexadecyl-oxirane	2912	—	—	0.44
64.	1-Tricosene	2936	—	13.41	—
65.	Cholest-5-en-3-ol(3β)	2946	0.63	—	—
66.	4,4-Dimethyl-2-oxazolidinethione	2970	—	0.21	—
67.	Stigmastan-3,5,22-trien	2981	—	0.61	—
68.	Cholesterol	2995	—	0.18	1.75
69.	Stigmasta-5,22-dien-3-ol	2995	0.99	3.51	4.04
70.	(23S.)-Ethylcholest-5-en-3-β-ol	2999	4.21	—	—
71.	n.-Triacontane	3000	2.35	—	—
72.	1-Hentetracontanol	3000	—	0.67	—
73.	2-(2′-Furyl)-3-phenyl-1H-indol	3019	—	—	0.21
74.	β-Amyrin	3019	5.53	—	—
75.	Campesterol	3033	0.97	2.04	1.49
76.	9,10-Dihyro-9,10,11-trimethyl-9,10-methanoanthracen-11-ol	3036	—	—	0.18
77.	Ergostanol	3036	—	0.09	—
78.	Stigmasterol	3048	—	0.22	3.09
79.	Lupenol	3057	1.11	—	—
80.	22,23-Dihydro-stigmasterol	3064	—	—	2.80
81.	α-Amyrin	3066	2.48	0.71	—
82.	γ-Sitosterol	3066	—	7.17	—
83.	23(Z.)-Ethylcholestanol	3068	—	0.85	—
84.	Δ-8(14)-Stigmasterol	3082	—	0.43	—
85.	(3β,5α)-Stigmast-7-en-3-ol	3082	0.27	—	—
86.	Stigmast-4-en-3-one	3108	—	—	0.93
87.	24-Methylenecycloartanol	3108	0.52	1.31	—
88.	Vitamin E	3111	0.44	0.55	—
89.	d,α.-Tocopherol	3111	0.44	0.34	—
90.	Moretenol	3120	11.71	—	—
91.	Friedelinol	3123	—	—	1.17
92.	6-Aza-β-homo-5-α-cholestano[6,7-d]tetrazole	3124	8.33	—	—
93.	Neophytadiene	3158	—	5.12	—
Total Identification %		82.64%	91.74%	69.05%

RI, retention indices on HP-5 capillary column.

Table 2.. Classification of lipid components of T.callosum..

	Chemical classes	Flower %	Stem %	Root %
1.	Terpenes and terpenoids	22.78	9.92	9.77
2.	Hydrocarbons	27.89	20.46	10.34
3.	Alcohols	1.28	5.79	0.35
4.	Acids	8.98	34.25	7.66
5.	Oxygen-containing compounds	0.15	0.94	9.52
6.	Steroids	16.86	16.74	11.01
7.	Aromatics	1.80	1.87	14.66
8.	Others	2.90	2.06	3.07
Total identification		82.64	91.74	69.05