In Vitro.–Enhanced Production of Podophyllotoxin in Phytohormonal-Induced and Regenerated Roots of Podophyllum hexandrum.

Figures & data

Table 1.. Root induction in Gamborg's B5 or Murashige and Skoog's (MS) media supplemented with various plant growth regulators.

Sample no.	Hormonal combinations in Gamborg's or MS media	Observations (B5 medium)	Observation (MS medium)
1	NAA (2 mg/l)	+	+
2	IAA (2 mg/l)	+ +	+
3	IBA (2 mg/l)	+	+
4	IAA (2 mg/l) + NAA (2 mg/l)	+ + +	+ +
5	IAA (2 mg/l) + NAA (5 mg/l)	+ +	+ +
6	IAA (0.003 mg/l) + NAA (0.003 mg/l)	+ +	+
7	IAA (1 mg/l) + NAA (2 mg/l)	+	+
8	IBA (2 mg/l) + NAA (2 mg/l)	+ +	+ +
9	NAA (2 mg/l) + 2,4-D (5 mg/l)	+	−
10	IAA (2 mg/l) + KIN (2 mg/l)	+	−
11	2,4-D (5 mg/l) + KIN (2 mg/l)	−	−
12	IAA (1 mg/l) + NAA (1 mg/l) + 2,4-D (2 mg/l)	+	−
13	IAA (1 mg/l) + NAA (1 mg/l) + 2,4-D (5 mg/l)	−	−
14	IAA (1 mg/l) + NAA (1 mg/l) + 2,4-D (3 mg/l)	+	−
15	IBA (2 mg/l) + NAA (2 mg/l) + IAA (2 mg/l)	+	+

+++, excellent root induction; ++, comparatively good root induction; +, normal root induction; −, no root induction.

Table 2.. Effects of plant growth regulators on root regeneration.

Sample no.	Hormonal combinations in Gamborg's and Murashige and Skoog's (MS) media	Observations (B5 medium)
1	Gamborg's B5 + IAA (2 mg/l)	+
2	Gamborg's B5 + IBA (2 mg/l) + IAA (1 mg/l)	+ +
3	Gamborg's B5 + IAA (2 mg/l) + NAA (2 mg/l)	+ + +
4	Gamborg's B5 + IBA (2 mg/l) + NAA (2 mg/l)	+ +
5	Gamborg's B5 + IAA (3 mg/l) + NAA (2 mg/l)	+ +
6	Gamborg's B5 + 6-BA (1 mg/l) + IAA (1 mg/l)	−
7	MS + IAA(2 mg/l) + NAA (2 mg/l)	+ +
8	MS + BA (2 mg/l) + NAA (2 mg/l)	+
9	MS + IAA (3 mg/l) + NAA (2 mg/l)	+
10	MS + 6-BA (1 mg/l) + NAA (1 mg/l)	−

+++, excellent root induction; ++, comparatively good root induction; +, normal root induction; −, no root induction.

Figure 1 Four-day-old aseptically germinated seedling of Podophyllum hexandrum. Royle.

Figure 2 Induction of rooting after 30 days in Gamborg's B5 medium supplemented with IAA (2 mg/l) + NAA (2 mg/l).

Figure 3 Induction of rooting after 30 days in Gamborg's B5 medium supplemented with IAA (2 mg/l) + NAA (2 mg/l).

Figure 4 Induction of rooting after 30 days in Gamborg's B5 medium supplemented with IAA (2 mg/l) + NAA (5 mg/l).

Figure 5 Induction of rooting after 30 days in Gamborg's B5 medium supplemented with IBA (2 mg/l) + NAA (2 mg/l).

Figure 6 Induction of rooting after 30 days in Gamborg's B5 medium supplemented with IBA (2 mg/l) only.

Figure 7 Root explant showing initiation of callus after 15 days in Gamborg's B5 medium supplemented with 2,4-D (2 mg/l) + KIN (0.5 mg/l).

Figure 8 Regeneration of roots from developed calli after 45 days in Gamborg's B5 medium supplemented with IAA (2 mg/l) + NAA (2 mg/l).