Figures & data
Figure 1 After the induction of arthritis, the arthritic mice were divided into two groups, namely, positive control group (olive alone) and test group (0.1 mg of PZE-6). Each group consisted of five animals. Treatment was done daily until the end of the experiment. The third group, nonarthritic mice, did not receive any injection. Arthritic index (AI) at 3-, 5-, and 7-week intervals after primary immunization was measured. The arithmetic mean from the measurements of all animals in the group was taken. The p value of groups receiving PZE-6 is < 0.05.
Figure 2 Retro-orbital sera were collected at 3-, 5-, and 7-week intervals after primary immunization for the estimation of anti-type II collagen antibody (IgG) by ELISA. The results presented are the means of triplicates with standard deviation of each serum dilution. The p value of groups receiving PZE-6 is < 0.05.
Figure 3 Retro-orbital sera were collected at 3-, 5-, and 7-week intervals after primary immunization for the estimation of IgG subclasses, namely, IgG1, IgG2a, IgG2b, and IgG3, by ELISA. The results presented are the mean of triplicates with standard deviation of each serum dilution. The p value of groups receiving PZE-6 is < 0.05.
Figure 4 After the induction of arthritis, the mice were divided into five groups, namely, control group (CFA alone), another group, treated with indomethacin, and three test groups (treated with 0.1, 0.2, 0.4 mg of PZE-6). Treatment was done daily until the end of the experiment. Each group consisted of five animals. Retro-orbital sera were collected at 3-, 5-, and 7-week intervals after primary immunization for the estimation of anti-type II collagen antibody (IgG) by ELISA. The results presented are the means of triplicates with standard deviation of each serum dilution. The p value of groups receiving PZE-6 is < 0.05.
Figure 5 After the induction of arthritis, mice were divided into two groups namely, test group, treated (i.p.) with PZE-6 (0.1 mg), and control group, treated with vehicle alone. The third group, nonarthritic mice, did not receive any injection. Spleenic T lymphocytes, isolated from arthritic mice (two groups) and nonarthritic normal mice (one group), were incubated with 1 and 2 µg of Con A. Each group consisted of five animals. After 18 h incubation of cell cultures, radioactivity was measured using scintillation counter. T cells without Con A stimulation served as blank. The results presented are the means of triplicates with standard deviation of each serum dilution. The p value of groups receiving PZE-6 is < 0.05.
