The Analgesic and Anti-Inflammatory Activities of the Extracts of Phyllanthus reticulatus. in Mice Model

Figures & data

Table 1.. Analgesic activity of different extracts of Phyllanthus reticulatus. on acetic acid-induced writhing response and radiant heat tail-flick model in mice.

	Acetic acid induced writhing response in mice			Radiant heat tail flick
Group	Dose^a. (mg/kg body weight)	Writhings^b.	% Inhibition	Reaction time (sec)^b.	% of elongation
Control	–	27.0±1.31	–	4.05±0.20	–
PHRPE	150	25.1±1.26	7.09	4.85±0.23	19.75
	300	24.5±1.06	9.25	4.97±0.29	22.63
PHREA	150	13.2±0.89**	51.23	5.58±0.86	37.86
	300	9.42±0.55**	65.12	5.77±0.65	42.38
PHRME	150	21.2±2.33*	21.60	5.57±0.29	37.44
	300	20.8±2.16*	22.83	6.5±0.28**	60.49
Aminopyrine	50	4.0±0.52**	85.18	–	–
Morphine	2^c.	–	–	7.97±0.63**	96.7
One-way ANOVA	F	36.0		5.82
	Df	7, 40		7, 40
	P.	<0.001		<0.05

^a.1 h after drug treatment, mice were injected i.p. with 0.7% (v/v) acetic acid (0.1 ml/10 g); immediately after the injection.

^b.The number of writhes was counted for 10 min; values are mean±SEM (n. = 6); one-way ANOVA; **P. < 0.01, *P. < 0.05 compared to control.

^c.Morphine, 2 mg/kg body weight was administered subcutaneously.

Table 2.. Anti-inflammatory activity of different extracts of Phyllanthus reticulatus. on carrageenan-induced rat paw edema.

		Carrageenan induced rat paw edema^b. Mean±SEM (% inhibition of paw volume)
Group	Dose (mg/kg)^a.	1 h	2 h	3 h	4 h	24 h
Control	–	72.8±1.46	95.2±2.37	113.0±2.16	121.0±2.71	68.2±2.08
PHRPE	150	65.0±0.7*	85.2±2.13*	99.0±1.64**	105.0±2.25**	65.2±1.53
		(10.71)	(10.5)	(12.69)	(13.83)	(4.39)
	300	63.6±1.5**	82.6±2.2**	97.0±1.79**	102.0±2.62**	63.0±1.64
		(12.63)	(13.23)	(14.46)	(15.65)	(7.62)
PHREA	150	59.8±1.83**	77.2±2.5**	90.4±1.66**	90.4±1.66**	64.0±1.82
		(17.85)	(18.9)	(20.28)	(25.53)	(6.15)
	300	55.6±2.01**	70.0±2.10**	83.6±1.60**	83.6±1.60**	63.8±1.74
		(23.62)	(24.36)	(26.27)	(31.13)	(6.45)
PHRME	150	56.4±2.44**	70.6±2.01**	84.0±1.95**	86.4±2.25**	65.4±2.29
		(22.52)	(25.84)	(25.92)	(28.23)	(4.10)
	300	50.2±2.8**	62.8±2.73**	70.4±2.01**	72.8±1.96**	63.4±3.66
		(31.04)	(34.03)	(37.91)	(40.03)	(7.03)
PBZ	80	46.6±1.57**	54.2±1.88**	65.6±2.14**	78.8±1.83**	55.8 ±2.15**
		(35.98)	(43.06)	(42.15)	(35.09)	(18.18)
One-way ANOVA	F	19.9	33.2	68.6	55.1	2.58
	df	7, 32	7, 32	7, 32	7, 32	7, 32
	P.	<0.0001	<0.0001	<0.0001	<0.0001	<0.05

^a.1 h after treatment of test compounds, p. o., carrageenan was administered in rat hind paw.

^b.Values are Mean±SEM (n. = 6); paw volume is expressed in change of height (in mm) of Hg bath (in parentheses, % inhibition of edema). One-way ANOVA; **P. < 0.01, *P. < 0.05 compared to control. PBZ = Phenylbutazone.