Figures & data
Figure 1 Isobologram showing the synergistic effect of combination of piperitone with nitrofurantoin on the inhibition of Enterobacter cloacae..
![Figure 1 Isobologram showing the synergistic effect of combination of piperitone with nitrofurantoin on the inhibition of Enterobacter cloacae..](/cms/asset/ec4178a3-5a1e-4772-b20c-ce3caced434b/iphb_a_221236_f0001_b.gif)
Figure 2 HPLC chromatograms of nitrofurantoin extracts from the samples after 18 h of incubation with noninoculated medium (a), E. cloacae. (b), or E. cloacae. containing 1 µL/mL of piperitone (c). The peak eluted at 5.8 min represents nitrofurantoin.
![Figure 2 HPLC chromatograms of nitrofurantoin extracts from the samples after 18 h of incubation with noninoculated medium (a), E. cloacae. (b), or E. cloacae. containing 1 µL/mL of piperitone (c). The peak eluted at 5.8 min represents nitrofurantoin.](/cms/asset/cb8a4d18-676c-4649-badd-c89e25b0dc84/iphb_a_221236_f0002_b.gif)
Figure 3 Nitrofurantoin remaining in Mueller-Hinton medium before incubation (bar 1), after 18 h of sterile incubation (bar 2) with E. cloacae. and 1 µl piperitone (bar 3), and with E. cloacae. only (bar 4). The areas of the peaks found by HPLC were measured by integration.
![Figure 3 Nitrofurantoin remaining in Mueller-Hinton medium before incubation (bar 1), after 18 h of sterile incubation (bar 2) with E. cloacae. and 1 µl piperitone (bar 3), and with E. cloacae. only (bar 4). The areas of the peaks found by HPLC were measured by integration.](/cms/asset/c1c843b4-1ef3-4614-b2ac-90c747f1ce00/iphb_a_221236_f0003_b.gif)
Table 1.. Growth of nitrofurantoin-resistant E. cloacae. in media containing a noninhibitory concentration of nitrofurantoin in the presence and absence of a subinhibitory concentration of piperitone.
Table 2.. Antibacterial activity of nitrofurantoin before and after incubation with Enterobacter cloacae. in the presence and absence of piperitone against a nitrofurantoin-sensitive strain of Escherichia coli..
Table 3.. Nitroreductase produced by nitrofurantoin-resistant E. cloacae. in the presence and absence of piperitone, units per Optimal Density unit of cells (A.600).