Antihepatotoxic Effect of Punica granatum. Acetone Extract Against Isoniazid- and Rifampicin-Induced Hepatotoxicity

Figures & data

Table 1.. Effect of Punica granatum. on the activities of marker enzymes in serum of control and experimental groups of rats.

Groups	GOT	GPT	LDH	ALP
Control	23.65 ± 2.30	33.93 ± 3.30	79.33 ± 7.91	47.45 ± 4.69
Antituberculosis drugs (isoniazid, rifampicin, pyrazinamide)	39.64 ± 3.86	55.45 ± 5.52	159.50 ± 15.52	87.39 ± 8.65
Punica granatum. alone	24.41 ± 2.41	34.12 ± 3.36	79.93 ± 7.21	47.52 ± 4.60
Punica granatum. + antituberculosis drugs (isoniazid and rifampicin)	26.45 ± 2.68	38.31 ± 3.84	88.98 ± 8.71	50.05 ± 5.02

Results are expressed as mean ± SD (n = 6). *p < 0.05.

Comparisons are made between group 1 (control) with group 2 (antituberculosis drug isoniazid- and rifampicin-, induced) and group 2 with group 4 [Punica granatum. + antituberculosis drugs (isoniazid and rifampicin)].

Activity is expressed as µmol pyruvate liberated per mg of protein per h for LDH, GOT, and GPT; IU/L for ALP.

Table 2.. Effect of Punica granatum. on the activities of antioxidant enzymes in the livers of control and experimental groups of rats.

Groups	GPx	GST	SOD	CAT
Control	3.63 ± 0.36	633.75 ± 63.36	3.61 ± 0.36	4.58 ± 0.46
Antituberculosis drugs (isoniazid and rifampicin)	1.66 ± 0.16	357.83 ± 35.12	1.66 ± 0.15	3.536 ± 0.35
Punica granatum. alone	3.43 ± 0.34	621.84 ± 62.35	3.56 ± 0.31	4.43 ± 0.43
Punica granatum. + antituberculosis drugs (isoniazid and rifampicin)	3.47 ± 0.34	633.73 ± 62.64	3.58 ± 0.35	4.46 ± 0.44

Results are expressed as mean ± SD (n = 6). *p < 0.05.

Comparisons are made between group 1 (control) with group 2 (antituberculosis drug isoniazid- and rifampicin-, induced) and group 2 with group 4 [Punica granatum. + antituberculosis drugs (isoniazid and rifampicin)].

Activity is expressed as: Unit min⁻¹ mg protein⁻¹ for GST; µmol GSH oxidized min⁻¹ mg protein⁻¹ for GPx; 50% inhibition of epinephrine autoxidation for SOD; µmol H₂O₂ decomposed min⁻¹ mg protein⁻¹ for CAT.

Figure 1 Level of glutathione in the livers of control and experimental groups of rats. Results are expressed as mean ± SD (n = 6). *p < 0.05. Comparisons are made between group 1 (control) with group 2 (antituberculosis drugs induced) and group 2 with group 4 (Punica granatum. + antituberculosis drugs).

Figure 2 Levels of vitamins C and E in the livers of control and experimental groups of rats. Results are expressed as mean ± SD (n = 6). *p < 0.05. Comparisons are made between group 1 (control) with group 2 (antituberculosis drugs induced) and group 2 with group 4 (Punica granatum. + antituberculosis drugs).

Figure 3 Levels of lipid peroxides in the livers of control and experimental groups of rats. Results are expressed as mean ± SD (n = 6). *p < 0.05. Comparisons are made between group 1 (control) with group 2 (antituberculosis drugs induced) and group 2 with group 4 (Punica granatum. + antituberculosis drugs).