Effect of Helicteres isora. Bark Extracts on Brain Antioxidant Status and Lipid Peroxidation in Streptozotocin Diabetic Rats

Figures & data

Table 1.. Effect of aqueous extracts of the bark of Helicteres isora. on blood glucose and plasma insulin in normal and experimental rats.

Group (n = 6)	Treatment	Fasting blood glucose (mg/dL)	Plasma insulin (µunit/mL)
I	Normal	85 ± 5^a	13 ± 0.8^a
II	Normal + HIBe (100 mg/kg)	79 ± 4^a	15 ± 1^b
III	Normal + HIBe (200 mg/kg)	74 ± 5^b	16 ± 3^b
IV	Diabetic control	270 ± 15^c	4 ± 0.3^c
V	Diabetic + HIBe (100 mg/kg)	115 ± 3^d	11 ± 4^d
VI	Diabetic + HIBe (200 mg/kg)	96 ± 3^e	9 ± 3^e
VII	Diabetic + tolbutamide (250 mg/kg)	98 ± 6^e	9 ± 1^e

Values are given as means ± SD of six animals in each group. Values not sharing a common superscript (a, b, c, d, and e) differ significantly at p < 0.05, Duncan's multiple range test (DMRT).

Table 2.. Effect of aqueous extracts of bark of Helicteres isora. on brain TBARS and hydroperoxides in normal and experimental rats.

Group (n = 6)	Treatment	TBARS (mM/100 g tissue)	Hydroperoxides (mM/100 g tissue)
I	Normal	1.12 ± 0.06^a	114.15 ± 3.20^a
II	Normal + HIBe (100 mg/kg)	1.04 ± 0.05^a	110.50 ± 2.70^a
III	Normal + HIBe (200 mg/kg)	0.98 ± 0.03^a	98.70 ± 3.05^b
IV	Diabetic control	1.98 ± 0.07^b	135.68 ± 2.45^c
V	Diabetic + HIBe (100 mg/kg)	1.25 ± 0.06^c	113.22 ± 3.26^d
VI	Diabetic + HIBe (200 mg/kg)	1.20 ± 0.03^c	110.42 ± 3.08^d
VII	Diabetic + tolbutamide (250 mg/kg)	1.36 ± 0.06^d	120.40 ± 4.05^e

Values are given as means ± SD of six animals in each group. Values not sharing a common superscript (a, b, c, d, and e) differ significantly at p < 0.05, Duncan's multiple range test (DMRT).

Table 3.. Effect of aqueous extract of bark of Helicteres isora. on brain CAT and SOD in normal and experimental rats.

Group (n = 6)	Treatment	CAT (Units^A/mg protein)	SOD (Units^B/mg protein)
I	Normal	3.25 ± 0.29^a	7.80 ± 0.40^a
II	Normal + HIBe (100 mg/kg)	3.85 ± 0.20^a	7.55 ± 0.32^a
III	Normal + HIBe (200 mg/kg)	4.05 ± 0.20^b	7.42 ± 0.35^a
IV	Diabetic control	0.76 ± 0.15^c	5.24 ± 0.15^b
V	Diabetic + HIBe (100 mg/kg)	2.95 ± 0.20^d	7.15 ± 0.38^c
VI	Diabetic + HIBe (200 mg/kg)	3.05 ± 0.45^d	7.48 ± 0.20^d
VII	Diabetic + tolbutamide (250 mg/kg)	2.35 ± 1.35^e	7.12 ± 0.30^c

Values are given as means ± SD of six animals in each group. Values not sharing a common superscript (a, b, c, d, and e) differ significantly at p < 0.05, Duncan's multiple range test (DMRT).

^Aµmole of H₂O₂ consumed/minute.

^BOne unit of activity was taken as the enzyme reaction, which gave 50% inhibition of NBT reduction in 1 min.

Table 4.. Effect of aqueous extract of bark of Helicteres isora. on brain GPx, GST, and GSH in normal and experimental rats.

Group (n = 6)	Treatment	GPx (Units^A/mg protein)	GST (Units^B/mg protein)	GSH (mg/100 g tissue)
I	Normal	3.48 ± 0.15^a	5.74 ± 0.15^a	35.25 ± 3.21^a
II	Normal + HIBe (100 mg/kg)	3.59 ± 0.18^a	5.75 ± 0.28^a	38.12 ± 2.14^a
III	Normal + HIBe (200 mg/kg)	3.66 ± 0.20^a	5.84 ± 0.20^a	39.18 ± 3.20^a
IV	Diabetic control	1.10 ± 0.25^b	1.24 ± 0.02^b	14.82 ± 1.39^b
V	Diabetic + HIBe (100 mg/kg)	2.85 ± 0.15^c	2.65 ± 0.13^c	25.16 ± 2.01^c
VI	Diabetic + HIBe (200 mg/kg)	3.24 ± 0.20^d	3.25 ± 0.20^d	32.08 ± 2.20^d
VII	Diabetic + tolbutamide (250 mg/kg)	2.47 ± 0.12^e	2.15 ± 0.16^e	25.50 ± 2.10^c

Values are given as means ± SD of six animals in each group. Values not sharing a common superscript (a, b, c, d, and e) differ significantly at p < 0.05, Duncan's multiple range test (DMRT).

^Aµg of GSH consumed/min.

^Bµmoles of CDNB-GSH conjugate formed/min.