Antimicrobial screening and phytochemical analysis of the resin part of Acacia catechu

Figures & data

Table 1.  Antimicrobial activity of Acacia catechu extracts (1 mg/mL).

Name of Solvent [Extract yield (%) in solvent]	Zone of inhibition (mm)
	E. coli	S. aureus	P. aeruginosa	B. subtilis
Petroleum ether (0.11)	6.75 ± 0.14	4.65 ± 0.19	3.44 ± 0.22	1.9 ± 0.14
Chloroform (0.05)	2.34 ± 0.27	2.85 ± 0.5	6.4 ± 0.4	3.08 ± 0.14
Methanol (31.1)	8.8 ± 1.57	4.5 ± 0.44	2.4 ± 0.4	1.25 ± 0.77
Water (36.8)	1.65 ± 0.22	6 ± 0.1	4.63 ± 0.22	8.42 ± 0.14

Table 2.  Minimum inhibitory concentration (MIC; in μg/mL) of Acacia catechu extracts against bacteria.

Solvent	MIC (μg/mL)
	E. coli	S. aureus	P. aeruginosa	B. subtilis
Petroleum ether	140	50	10	40
Chloroform	820	30	50	160
Methanol	60	140	190	690
Water	330	40	220	20

Figure 1.  Chromatograms obtained with different extracts of Acacia catechu. TLC synopsis: (A) UV lamp image at 254 nm; (B) Mercuric lamp image at 254 nm; and (C) White lamp image at 400- 800 nm. Five spots of five different extracts were made. Spot abbreviations are described below with their application volume:

a = ethyl acetate extract 15 μL, b = methanol extract 15 μL, c = petroleum ether extract 15 μL, d = butanol extract 15 μL, e = chloroform extract 15 μL. Catechin/epicatechin is visible at R_f value of ∼ 0.15 and quercetin at 0.6-0.7.