Cytoprotective activity of amifostine on HeLa cell cultures treated with daunorubicin and evaluation of gene expression by RT-PCR

Figures & data

Table 1.  Effect of AMI and DAU either alone or in combination for 24 and 48 h in HeLa cells, given in ± SD.

Treatment groups	Absorbance value (570 nm)
	24 h	48 h
Control	10 ± 1.68 × 10^−2	11 ± 1.53 × 10^−2
AMI	9 ± 0.54 × 10^−2	10 ± 0.88 × 10^−2
DAU	4.2 ± 0.84 × 10^−2*	2.4 ± 0.33 × 10^−2*
AMI + DAU	5.0 ± 0.66 × 10^−2*	2.0 ± 0.43 × 10^−2*

*p <0.001.

Figure 1.  Cell growth inhibition of HeLa cell lines by AMI, DAU and AMI+DAU treatment for 24 and 48 h.

Table 2.  Mitotic index values of treated cells, given in ± SD.

Treatment groups	24 h	48 h
Control	3.75 ± 0.66	4.36 ± 0.56
AMI	3.99 ± 0.09	4.03 ± 0.77
DAU	2.12* ± 0.01	1.24* ± 0.37
AMI + DAU	2.44* ± 0.22	1.87* ± 0.02

*p <0.05 (according to control).

Table 3.  Apoptotic cell number of drugs in HeLa cell lines.

Treatment groups	24 h	48 h
Control	4 ± 0.88	6 ± 1.44
AMI	3 ± 1.03	7 ± 1.97
DAU	28* ± 2.06	46* ± 2.48
AMI + DAU	30* ± 3.99	44* ± 4.01

*p <0.05 (according to control).

Figure 2.  Apoptotic index of HeLa cell lines treated with drugs for 24 and 48 h (respectively).

Figure 3.  DNA profiles of AMI, DAU and AMI+DAU treatment groups in HeLa cells for 24 and 48 h (M, Marker; C, Control; 1, AMI; 2, DAU (↓) and 3, AMI+DAU for 24 h; C, Control; 4, AMI; 5, DAU (↓) and 6, AMI+DAU for 48 h).

Figure 4.  Bcl-2 expression of HeLa cells (M, Marker; C, Control; 1, AMI; 2, DAU and 3, AMI+DAU for 24 h; C, Control; 4, AMI; 5, DAU and 6, AMI+DAU for 48 h).

Figure 5.  Bax expression of HeLa cells (M, Marker; C, Control; 1, AMI; 2, DAU and 3, AMI+DAU for 24 h; C, Control; 4, AMI; 5, DAU and 6, AMI+DAU for 48 h).